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Fluorescence changes in human gamma-globulin induced by free-radical activity



Fluorescence changes in human gamma-globulin induced by free-radical activity



Biochimica et Biophysica Acta 742(3): 607-616



Low-intensity ultraviolet irradiation was used to generate free-radical activity in lipid-free human gamma-globulin. The changes were monitored by fluorescence spectroscopy and gel filtration chromatography. The pattern and the effect of thiol compounds, free-radical scavengers and antioxidants point to a free-radical-mediated process. Initial energy uptake was probably in the region of aromatic amino acid residues, followed by complex intramolecular rearrangements. Irradiation led to the formation of molecular species and complexes whose fluorescence characteristics were different from those of native gamma-globulin and indistinguishable from those observed in inflammatory exudates.

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Accession: 043137007

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PMID: 6188491

DOI: 10.1016/0167-4838(83)90279-0


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