+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Forensic DNA typing using AmpliType PM (Amp-PM) kit--allele frequency distributions of the five marker loci in Japanese population, and evaluation of Amp-PM markers for typing of saliva stain and hair



Forensic DNA typing using AmpliType PM (Amp-PM) kit--allele frequency distributions of the five marker loci in Japanese population, and evaluation of Amp-PM markers for typing of saliva stain and hair



Nihon Hoigaku Zasshi 49(3): 199-204



Population study was carried out on the Japanese from Toyama district using the AmpliType PM PCR amplification and typing kit (Perkin Elmer) system, and then application of the kit system to forensic materials was evaluated. Using the kit system, 2 alleles could be identified for every locus of low density lipoprotein receptor (LDLR), glycophorin A (GYPA), hemoglobin G gammaglobin (HBGG), D7S8, and 3 alleles for group specific component (GC) in a sample size of 100 unrelated individuals. Allele frequency distributions of the sample were 0.18 for A, 0.82 for B in LDLR; 0.61 for A, 0.39 for B in GYPA; 0.27 for A, 0.73 for B, 0 for C in HBGG; 0.61 for A, 0.39 for B in D7S8 and 0.31 for A, 0.48 for B, 0.21 for C in GC. Deviations from Hardy-Weinberg equilibrium could not be observed in LDLR, GYPA, D7S8 and GC loci, except in HBGG locus (p < 0.05). All genotypes of the 5 loci could be detected from every saliva stains from 12 donors, but the genotypes estimated from 3 saliva samples unexpectedly did not correspond to those on the blood from same donors. Identification of the genotype from a hair root in telophase was available only in 3 individual samples out of 12 donors. The present experiment show that further improvement should be made on the application of the kit system to saliva stain.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 043145371

Download citation: RISBibTeXText

PMID: 7674560


Related references

Allele frequency distributions of five loci (LDLR, GYPA, HBGG, D7S8 and GC) in a Japanese population: Application of AmpliType PM Kit to paternity testing. Journal of Saitama Medical School 23(1): 61-75, 1996

A powerful, novel, multiplex typing system for six short tandem repeat loci and the allele frequency distributions in two Japanese regional populations. Electrophoresis 23(19): 3332-3340, 2002

The STR-marker FGA Allele frequency data , automated typing with different fluorescence markers. Olaisen, B , Brinkmann, B , Lincoln, P J International Congress Series; Progress in Forensic Genetics, 7 460-462, 1998

Typing of three STRs loci Development of a multiplex PCR system for forensic samples and parentage testing and allele frequency distribution in Tuscany region. Olaisen, B , Brinkmann, B , Lincoln, P J International Congress Series; Progress in Forensic Genetics, 7 341-343, 1998

A novel fluorescent quadruplex STR typing system and the allele frequency distributions in a Thai population. Journal of Forensic Sciences 48(1): 116-121, 2003

Optimization of short tandem repeats (STR) typing method and allele frequency of 8 STR markers in referring to forensic medicine of Semnan Province. Journal of Medicine and Life 8(Spec Iss 4): 180-185, 2015

Allele frequency distributions for nine STR loci in the Japanese population. Journal of Forensic Sciences 44(6): 1319, 1999

Allele frequencies and haplotypic associations defined by allelic DNA typing at HLA class I and class II loci in the Japanese population. Tissue Antigens 56(6): 522-529, 2000

Allele frequency distributions at several variable number of tandem repeat (VNTR) and short tandem repeat (STR) loci in a restricted Caucasian population from south Italy and their evaluation for paternity and forensic use. Molecular and Cellular Probes 10(4): 299-308, 1996

Forensic animal DNA typing: Allele nomenclature and standardization of 14 feline STR markers. Forensic Science International. Genetics 12: 42-59, 2015

A proposal for standardization in forensic bovine DNA typing: allele nomenclature of 16 cattle-specific short tandem repeat loci. Animal Genetics 40(5): 630-636, 2010

Report of the blind trial of the Cetus Amplitype HLA DQ alpha forensic deoxyribonucleic acid (DNA) amplification and typing kit. Journal of Forensic Sciences 36(5): 1551-1556, 1991

Summary of validation studies from twenty-six forensic laboratories in the United States and Canada on the use of the AmpliType PM PCR amplification and typing kit. Journal of Forensic Sciences 42(1): 39-48, 1997

A proposal for standardization in forensic canine DNA typing: allele nomenclature of six canine-specific STR loci. Journal of Forensic Sciences 51(2): 274-281, 2006

A proposal for standardization in forensic equine DNA typing: allele nomenclature for 17 equine-specific STR loci. Animal Genetics 41(2): 122-127, 2010