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Gas chromatographic determination of free amino acids and amino acids attached to transfer RNA in biological samples

Lindqvist, L.; Mäenpää, P.H.

Journal of Chromatography 232(2): 225-235

1982

ISSN/ISBN: 0021-9673
PMID: 7153274
DOI: 10.1016/s0378-4347(00)84163-8
Accession: 043176497
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A method was developed to analyze quantitatively free amino acids and amino acids attached to transfer RNA (tRNA) in tissue samples by gas chromatography. Free amino acids were purified by ion-exchange chromatography after deproteinization. Total cellular aminoacyl-tRNA was extracted from rabbit reticulocytes and liver by a modified phenol extraction method under conditions which were designed to prevent deacylation of the attached amino acids. After deacylation and separation from tRNA by pressure ultrafiltration, eighteen amino acids were determined by gas chromatography as their N-heptafluorobutyryl isobutyl derivatives.

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Related References

Lindqvist, L.K.; Maempaa, P.H. 1981: Determination of free amino acids and amino acids attached to transfer rna in tissues by gas liquid chromatography Biochemical Society Transactions 9(2): 201P
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