Section 44
Chapter 43,184

Gene expression in bacteriophage lambda. II. Regulation of RNA synthesis in vivo by the N protein

Konrad, M.

Journal of Molecular Biology 53(3): 389-400


ISSN/ISBN: 0022-2836
PMID: 5493281
DOI: 10.1016/0022-2836(70)90073-2
Accession: 043183068

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Various mutant lysogens, all containing a temperature-sensitive repressor, were induced and the rate of synthesis of viral Rna transcribed to the left as well as to the right was measured. We have come to the following conclusions. (1) At very early times after induction both L and R viral RNA‡ are co-ordinately induced with a ratio of approximately five to one by weight. (2) Mutations in gene N greatly depress viral Rna synthesis, while mutations in genes O and P depress Rna synthesis only slightly. (3) The continued activity, even after development has started, of the N protein is shown to be necessary for normal viral Rna synthesis.

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