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Glutamine synthetase cascade: enrichment of uridylyltransferase in Escherichia coli carrying hybrid ColE1 plasmids


, : Glutamine synthetase cascade: enrichment of uridylyltransferase in Escherichia coli carrying hybrid ColE1 plasmids. Archives of Biochemistry and Biophysics 203(1): 181-189



Accession: 043202449

PMID: 6105849

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Related references

Garcia, E.; Federici, M.; Rhee, S.G.; Berberich, M.A., 1980: Glutamine synthetase ec 6.3.1.2 cascade enrichment of uridylyl transferase in escherichia coli carrying hybrid col e 1 plasmids. Hybrid ColE1 plasmids that carry small selected segments of the E. coli chromosome were used to obtain increased levels of 2 of the components involved in the covalent modification of glutamine synthetase. Screening of the plasmid collection for t...

Garcia, E.; Rhee, S.G., 1983: Cascade control of Escherichia coli glutamine synthetase. Purification and properties of PII uridylyltransferase and uridylyl-removing enzyme. Uridylyltransferase, a component of the covalent modification cascade system that controls glutamine synthetase activity in Escherichia coli, has been purified to apparent homogeneity. The purification was facilitated by the use of an E. coli stra...

Adler, S.P.; Purich, D.; Stadtman, E.R., 1975: Cascade control of Escherichia coli glutamine synthetase. Properties of the PII regulatory protein and the uridylyltransferase-uridylyl-removing enzyme. The PII regulatory protein of Escherichia coli glutamine synthetase exists in two interconvertible forms: a uridylylated form (PIID) which promotes the deadenylylation of glutamine synthetase and an unmodified form (PIIA) which promotes the adenyl...

Wickner, S.H.; Wickner, R.B.; Raetz, C.R., 1976: Overproduction of DNA gene products by Escherichia coli strains carrying hybrid ColE1 plasmids. Biochemical and Biophysical Research Communications 70(2): 389-396

Covarrubias, A.A.; Sánchez-Pescador, R.; Osorio, A.; Bolivar, F.; Bastarrachea, F., 1980: ColE1 hybrid plasmids containing Escherichia coli genes involved in the biosynthesis of glutamate and glutamine. Plasmid 3(2): 150-164

Vapnek, D.; Alton, N.K.; Bassett, C.L.; Kushner, S.R., 1976: Amplification in Escherichia coli of enzymes involved in genetic recombination: construction of hybrid ColE1 plasmids carrying the structural gene for exonuclease I. Endo-R-HindIII restriction endonuclease fragments obtained from F30 and pMB9 plasmid DNAs were ligated in vitro and used to transform a recB21 recC22 sbcB15 strain of E. coli K-12. The inability of this strain to stably maintain pMB9 alone permitt...

Rhee, S.G.; Park, S.C.; Koo, J.H., 1985: The role of adenylyltransferase and uridylyltransferase in the regulation of glutamine synthetase in Escherichia coli. The regulation of GS activity involves two nucleotidylation cycles, the uridylylation cycle of PII and the adenylylation cycle of GS, which are catalyzed by two converter enzymes, uridylyltransferase and adenylyltransferase, respectively. The conv...

Magasanik, B.; Bueno, R., 1985: The role of uridylyltransferase and PII in the regulation of the synthesis of glutamine synthetase in Escherichia coli. Current Topics in Cellular Regulation 27: 215-220

Koo J.H.; Park S.C.; Rhee S.G., 1985: Effects of uridylyltransferase and adenylyltransferase on the synthesis of glutamine synthetase in escherichia coli. Federation Proceedings 44(3): 682

Nishimura, Y.; Takeda, Y.; Nishimura, A.; Suzuki, H.; Inouye, M.; Hirota, Y., 1977: Synthetic ColE1 plasmids carrying genes for cell division in Escherichia coli. Plasmid 1(1): 67-77