+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

GnRH-receptors of rat pituitary is a glycoprotein: differential effect of neuraminidase and lectins on agonists and antagonists binding



GnRH-receptors of rat pituitary is a glycoprotein: differential effect of neuraminidase and lectins on agonists and antagonists binding



Molecular and Cellular Endocrinology 26(1-2): 217-222



The interaction of 125I-labeled gonadotropin releasing hormone (GnRH) agonist, [D-Ser-(t-Bu)6,des-Gly10-ethylamide]-GnRH, and antagonist [D-pGlu1,D-Phe2,D-Trp3,6]-GnRH with rat pituitary membranes was studied. Their binding was affected differently by pretreatment of membranes with neuraminidase and by wheat-germ agglutinin. Pretreatment of the membranes with neuraminidase abolished the specific binding of both antagonist and agonist, in a dose-response manner, with the former being less affected. Wheat-germ agglutinin affected antagonist binding slightly (22% inhibition at 40 microgram/ml) but inhibited agonist binding more markedly (50% at 40 microgram/ml). This inhibitory effect was specific since it was readily reversed by N-acetylglucosamine. Other lectins, such as concanavalin A and soybean agglutinin affected the binding of both agonist and antagonist to only a small degree. These results suggest that the GnRH-receptor of rat pituitary is a glycoprotein which contains sialic acid residue and that GnRH agonists and antagonists bind differently to the same receptor.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 043205285

Download citation: RISBibTeXText

PMID: 6282672


Related references

GnRH-Receptor of rat pituitary is a glycoprotein: Differential effect of neuraminidase and lectins on agonists and antagonists binding. Molecular and Cellular Endocrinology 26(1): 217-222, 1982

Gonadotropin releasing hormone receptor of rat pituitary is a glyco protein differential effect of neuraminidase and lectins on agonists and antagonists binding. Molecular & Cellular Endocrinology 26(1-2): 217-222, 1982

Effect of dopamine agonists and antagonists on the binding of tritiated estradiol to its receptors in the anterior pituitary gland of male rats. Endocrinology 117(5): 1742-1748, 1985

Differential binding of agonists and antagonists to GABAA and glycine receptors. Acta Physiologica Hungarica 75 Suppl: 201-202, 1990

Alpha-Noradrenergic receptors in the brain: differential effects of sodium on binding of [3H]agonists and [3H]antagonists. Brain Research 140(2): 378-384, 1978

Pretreatment with gonadotropin-releasing hormone (GnRH) antagonists to prevent the flare-up effect of long-acting GnRH agonists: results of a pilot study. Fertility and Sterility 91(2): 647-648, 2009

Effect of pH on binding of agonists and antagonists to rat heart muscarinic receptors. Biochemical Journal 216(1): 11-19, 1983

Binding kinetics of the long-acting gonadotropin-releasing hormone (GnRH) antagonist antide to rat pituitary GnRH receptors. Endocrinology Philadelphia 135(1): 45-52, 1994

Differential effect of humoral endorphin on the binding of opiate agonists and antagonists. European Journal of Pharmacology 81(2): 227-236, 1982

Differential effect of a lanthanide on binding of muscarinic receptor agonists and antagonists. Pharmacologist 22(3): 165, 1980

Gonadotropin-releasing hormone (GnRH) agonists and GnRH antagonists do not alter endogenous GnRH secretion in short-term castrated rams. Endocrinology 127(5): 2523-2529, 1990

Effect of acetylation on the binding of agonists and antagonists to beta adrenergic receptors. Proceedings of the Australian Biochemical Society 10: 84, 1977

Effect of GnRH pulse frequency on pituitary concentrations of LH and FSH, GnRH receptors , gonadotropin subunit mRNA, and GnRH-R mRNA in anestrous cows. Journal of Animal Science 74(Suppl. 1): 231, 1996

The effect of duration of nutritional anestrus and GnRH treatment on pituitary LH, FSH, and GnRH receptors in cows. Journal of Animal Science 75(Suppl. 1): 218, 1997

Characterization of active and inactive states of CB1 receptor and the differential binding state modulation by cannabinoid agonists, antagonists and inverse agonists. Neuropharmacology 58(8): 1215-1219, 2010