+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Homologous pairing of single-stranded circular DNAs catalyzed by recA protein



Homologous pairing of single-stranded circular DNAs catalyzed by recA protein



Nucleic Acids Research 12(15): 6127-6139



RecA protein catalyzes annealing between pairs of circular single-stranded DNA molecules containing complementary sequences varying in length from 3550 nucleotides to 181 nucleotides. The reaction requires ATP and catalytic amounts of recA protein. Molecules containing large complementary inserts are annealed by recA protein to form large multimeric aggregates that migrate slowly in agarose gels. In contrast the products formed from circular molecules containing short complementary regions are principally dimeric structures. We have used electron microscopy, thermal denaturation and kinetic studies to analyze these reaction products. Our results indicate that recA protein catalyzes multiple nucleation events between complementary DNA sequences in the absence of a free end and when these sequences are flanked by extensive noncomplementary regions.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 043276977

Download citation: RISBibTeXText

PMID: 6382164

DOI: 10.1093/nar/12.15.6127


Related references

RecA protein promoted homologous pairing in vitro. Pairing between linear duplex DNA bound to HU Protein (nucleosome cores) and nucleoprotein filaments of recA protein-single-stranded DNA. Journal of Biological Chemistry 264(29): 17395-17400, 1989

Homologous pairing of single-stranded DNA and superhelical double-stranded DNA catalyzed by RecO protein from Escherichia coli. Journal of Bacteriology 177(3): 566-572, 1995

Three phases in homologous pairing: polymerization of recA protein on single-stranded DNA, synapsis, and polar strand exchange. Cold Spring Harbor Symposia on Quantitative Biology 47 Pt 2: 821-828, 1983

Homologous pairing between nucleosome cores on a linear duplex DNA and nucleoprotein filaments of RecA protein-single stranded DNA. Biochimie 73(2-3): 187-190, 1991

RecA protein-promoted homologous pairing and strand exchange between intact and partially single-stranded duplex DNA. Journal of Molecular Biology 223(1): 79-93, 1992

Homologous pairing in genetic recombination. The pairing reaction catalyzed by Escherichia coli recA protein. Journal of Biological Chemistry 256(14): 7565-7572, 1981

Homologous pairing in genetic recombination: recA protein makes joint molecules of gapped circular DNA and closed circular DNA. Cell 20(1): 223-235, 1980

Zinc-free and reduced T4 gene 32 protein binds single-stranded DNA weakly and fails to stimulate UvsX-catalyzed homologous pairing. Journal of Biological Chemistry 269(4): 2773-2781, 1994

An interaction between a specified surface of the C-terminal domain of RecA protein and double-stranded DNA for homologous pairing. Journal of Molecular Biology 274(2): 213-221, 1998

Specific defects in double-stranded DNA unwinding and homologous pairing of a mutant RecA protein. FEBS Letters 477(1-2): 129-134, 14 July, 2000

Homologous pairing between single-stranded DNA immobilized on a nitrocellulose membrane and duplex DNA is specific for RecA activity in bacterial crude extract. Nucleic Acids Research 21(16): 3653-3657, 1993

Hydrolysis of Atp Dependent on Homologous Double-Stranded Dna and Single-Stranded Fragments Promoted by RecA Protein of Escherichia coli11. The Journal of Biochemistry 91(5): 1767-1775, 1982

Hydrolysis of ATP dependent on homologous double-stranded DNA and single-stranded fragments promoted by RecA protein of Escherichia coli. Journal of Biochemistry 91(5): 1767-1775, 1982

Defective homologous pairing and proficient processive unwinding by the recA430 mutant protein and intermediates of homologous pairing by recA protein. Journal of Biological Chemistry 264(35): 21167-21176, 1989

Early stages in recA protein-catalyzed pairing: Analysis of coaggregate formation and non-homologous DNA contacts. Journal of Molecular Biology 228(2): 409-420, 1992