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Identification of wild-type or mutant alleles of bacterial genes cloned on a bacteriophage lambda vector: isolation of uvrC (am) and other mutants

Auerbach, J.I.; Howard-Flanders, P.

Journal of Bacteriology 146(2): 713-717

1981

ISSN/ISBN: 0021-9193
PMID: 6452445
Accession: 043319501
We have identified lambda transducing bacteriophages carrying deoxyribonucleic acid repair or recombination genes of Escherichia coli K-12 by their ability to infect and express their bacterial genes in mutant cells in an agar overlay. This technique has been used to recognize transducing phages carrying uvrC+, ssb+, and other genes and to isolate phages carrying mutant alleles unable to complement ssb or uvrC cells. Several uvrC mutations were obtained which were suppressor sensitive.

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Related References

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