+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Immunogenicity and antigenicity of human coronaviruses 229E and OC43



Immunogenicity and antigenicity of human coronaviruses 229E and OC43



Infection and Immunity 32(3): 1000-1006



The immunogenicity and antigenicity of human coronaviruses 229E and OC43 were studied by quantitative immunoelectrophoresis. Three distinct antigens were recognized in both coronavirus strains when T-100-solubilized whole virus was tested by two-dimensional immunoelectrophoresis against homologous rabbit antiserum. No antigens cross-reacted between strains, but the electrophoretic patterns against homologous antiserum were highly similar in that both strains had one electrophoretically fast antigen, one of intermediate mobility, and one of slow mobility. Immunization of rabbits with 10(9) plaque-forming units of virus was required for production of antiserum which recognized the three antigens; lesser amounts gave rise to antisera which recognized only one or two components. Precipitin lines excised from two-dimensional immunoelectropherograms were used successfully as immunogens to prepare monospecific antiserum to each of the antigens of OC43 and 229E. Monospecific antiserum to the slow component of 229E neutralized 229E only, and monospecific antiserum to the slow component of OC43 both neutralized and inhibited hemagglutination of OC43 virus. Human convalescent sera which possessed both complement-fixing and neutralizing antibody also recognized the slow-moving component.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 043333067

Download citation: RISBibTeXText

PMID: 6166561


Related references

Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies. Nan Fang Yi Ke Da Xue Xue Bao 26(3): 290-293, 2006

Antibodies to human coronaviruses 229E and OC43 in the population of C.R. Acta Virologica 34(4): 346-352, 1990

Polypeptides and functions of antigens from human coronaviruses 229E and OC43. Infection and Immunity 35(2): 515-522, 1982

Studies with human coronaviruses. II. Some properties of strains 229E and OC43. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 139(3): 722-727, 1972

Clinical impact of human coronaviruses 229E and OC43 infection in diverse adult populations. Journal of Infectious Diseases 208(10): 1634-1642, 2013

Direct diagnosis of human respiratory coronaviruses 229E and OC43 by the polymerase chain reaction. Journal of Virological Methods 97(1/2): 59-66, 2001

Antigenic characterization of human coronaviruses 229E and OC43 by enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 20(2): 175-180, 1984

Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43. Molecular and Cellular Probes 8(5): 357-364, 1994

Infection of primary cultures of human neural cells by human coronaviruses 229E and OC43. Journal of Virology 71(1): 800-806, 1997

Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture. Journal Of Virological Methods. 72(2): 145-152, E, 1998

Rises in titers of antibody to human coronaviruses OC43 and 229E in Seattle families during 1975-1979. American Journal of Epidemiology 123(5): 862-868, 1986

Detection of antibodies to human coronaviruses 229E and OC43 in the sera of multiple sclerosis patients and normal subjects. Infection and Immunity 41(1): 426-429, 1983

Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43. Journal of Infectious Diseases 191(12): 2033-2037, 2005

Survival of human coronaviruses 229E and OC43 in suspension and after drying onsurfaces: a possible source ofhospital-acquired infections. Journal of Hospital Infection 46(1): 55-60, 2000

Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43. Journal of Veterinary Science 11(2): 165-167, 2010