Section 44
Chapter 43,379

Independent expression of common and private tumor-specific cell surface antigens in somatic cell hybrids between Rous sarcoma virus-transformed and normal cells

Kuzumaki, N.; Oikawa, T.; Yamada, T.

Journal of the National Cancer Institute 72(2): 387-393


ISSN/ISBN: 0027-8874
PMID: 6319792
Accession: 043378167

Somatic cell hybrids were prepared by fusion of a Rous sarcoma virus (RSV)-induced mouse tumor (CSA1M cl-11) with a Chinese hamster embryo lung fibroblast line (Wg3h X AGr X Ouar). Some hybrid clones showed rapid anchorage-independent growth and intracellular production of RSV-pp60src. Other hybrid clones showed slow anchorage-dependent growth and no production of such a protein. The former expressed both a common tumor-specific cell surface antigen(s) (TSSA) shared with other RSV-induced tumors and a private TSSA not shared with any other tumors; however, the latter lost the common TSSA and expressed only the private TSSA. These results suggest that the common and private TSSA on CSA1M cl-11 cells are independent genetic traits and only the common TSSA is coordinated with other expressions of the RSV src gene phenotype.

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