+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Induction of premature termination of transcription of the mouse beta-globin gene by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB)



Induction of premature termination of transcription of the mouse beta-globin gene by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB)



Nucleic Acids Research 9(14): 3307-3319



Hybridization of pulse-labeled RNA from DRB-treated Friend cells to mouse beta-globin cDNA revealed that the appearance of beta-globin mRNA in the cytoplasm was inhibited by greater than 87%. To examine the effect of DRB (125 microM) on HnRNA synthesis, nuclear RNA was electrophoresed in methyl mercuric hydroxide gels, transferred to nitrocellulose, and hybridized with beta-globin specific probes. Full-length nuclear transcripts, while present in untreated cells, were not detected in DRB-treated cells. Using restriction enzymes, the cloned beta-globin gene was divided into fragments proceeding from the 5' gene region to the 3' gene region. RNA labeled in vitro by transcription in nuclei isolated from DRB-treated cells hybridized only to the promoter proximal DNA fragment. Transcripts hybridizing to fragments from both the 5' and 3' regions of the gene were produced in nuclei from untreated cells. Together these results indicate that DRB causes premature termination of transcription within the beta-globin gene.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 043387621

Download citation: RISBibTeXText

PMID: 6269079


Related references

Induction of premature termination of transcription of the mouse -globin gene by 5,6-dichloro-1--D-ribofuranosylbenzimidazole (Drb). Nucleic Acids Research 9(14): 3307-3319, 1981

5,6-dichloro-1-beta-ribofuranosylbenzimidazole enhances premature termination of late transcription of simian virus 40 DNA. Proceedings of the National Academy of Sciences of the United States of America 77(6): 3297-3301, 1980

Site of premature termination of late transcription of simian virus 40 DNA: enhancement by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. Proceedings of the National Academy of Sciences of the United States of America 79(9): 2743-2747, 1982

5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits transcription of the beta-hemoglobin gene in vivo at initiation. Journal of Biological Chemistry 262(28): 13697-13705, 1987

Multiple discrete sites for premature RNA chain termination late in adenovirus-2 infection: enhancement by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. Proceedings of the National Academy of Sciences of the United States of America 76(6): 2571-2575, 1979

Characterization of 3 transcription termination signals in the mouse beta globin gene. Journal of Cellular Biochemistry Suppl. (15 Part G): 263, 1991

Transcription termination within the E1A gene of adenovirus induced by insertion of the mouse beta-major globin terminator element. Cell 40(4): 897-905, 1985

Early termination of heterogeneous nuclear RNA transcripts in mammalian cells: accentuation by 5,6-dichloro 1-beta-D-ribofuranosylbenzimidazole. Proceedings of the National Academy of Sciences of the United States of America 76(11): 5750-5754, 1979

Transcription termination occurs within a 1000 base pair region downstream from the poly(A) site of the mouse beta-globin (major) gene. Nucleic Acids Research 12(22): 8723-8731, 1984

A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene. Proceedings of the National Academy of Sciences of the United States of America 84(23): 8306-8310, 1987

5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits transcription elongation by RNA polymerase II in vitro. Journal of Biological Chemistry 264(4): 2250-2257, 1989

Mechanism of action of dichloro-beta-D-ribofuranosylbenzimidazole: effect on in vitro transcription. Proceedings of the National Academy of Sciences of the United States of America 79(10): 3167-3170, 1982

Rapidly phosphorylated histone-like proteins are modulated in the course of transcription block by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. European Journal of Cell Biology 31(2): 354-359, 1983

Transcription termination at the chicken beta H-globin gene. Molecular and Cellular Biology 8(12): 5369-5377, 1988

The transcriptional elongation inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits transcription factor IIH-associated protein kinase. Journal of Biological Chemistry 270(41): 23922-5, 1995