+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid

Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid

Journal of Virology 33(1): 449-462

The two envelope glycoproteins and the viral nucleocapsid of the coronavirus A59 were isolated by solubilization of the viral membrane with Nonidet P-40 at 4 degrees C followed by sucrose density gradient sedimentation. Isolated E2 consisted of rosettes of peplomers, whereas E1, the membrane glycoprotein, was irregular and amorphous. Under certain conditions significant interactions occurred between components of Nonidet P-40-disrupted virions. Incubation of the Nonidet P-40-disrupted virus at 37 degrees C resulted in formation of a complex between one of the viral glycoproteins, E1, and the viral nucleocapsid. This was caused by a temperature-dependent conformational change in E1, resulting in aggregation of E1 and interaction with the viral RNA in the nucleocapsid. E1 also bound rRNA. The E1-nucleocapsid complexes can be distinguished on sucrose and Renografin density gradients from native viral nucleocapsids. The separation of the membrane glycoprotein E1 from the peplomeric glycoprotein E2 permitted preparation of antisera against these isolated proteins. A model is proposed for the arrangement of the three major structural proteins in the coronavirus A59 virion in relation to the viral envelope and RNA.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 043483023

Download citation: RISBibTeXText

PMID: 6245243

Related references

Nucleocapsid-independent assembly of coronavirus-like particles by co-expression of viral envelope protein genes. Embo Journal 15(8): 2020-2028, 1996

Interaction of viral envelope glycoproteins with fibronectin. Infection and Immunity 40(3): 876-881, 1983

The missing link in coronavirus assembly. Retention of the avian coronavirus infectious bronchitis virus envelope protein in the pre-Golgi compartments and physical interaction between the envelope and membrane proteins. Journal of Biological Chemistry 276(20): 17515-17523, 2001

Nucleocapsid amino acids 211 to 254, in particular, tetrad glutamines, are essential for the interaction between the nucleocapsid and membrane proteins of SARS-associated coronavirus. Journal of Microbiology 44(5): 577-580, 2006

Characterization of a critical interaction between the coronavirus nucleocapsid protein and nonstructural protein 3 of the viral replicase-transcriptase complex. Journal of Virology 87(16): 9159-9172, 2013

Nucleocapsid-independent specific viral RNA packaging via viral envelope protein and viral RNA signal. Journal of Virology 77(5): 2922-2927, 2003

Interactions between coronavirus nucleocapsid protein and viral RNAs: implications for viral transcription. Journal of Virology 62(11): 4280-4287, 1988

Interaction of coronavirus nucleocapsid protein with the 5'- and 3'-ends of the coronavirus genome is involved in genome circularization and negative-strand RNA synthesis. Febs Journal 286(16): 3222-3239, 2019

Molecular interactions of hepatitis B virus nucleocapsid and envelope peptides with HLA-DR1, -DR3, -DR7 and -DR13 glycoproteins. Hepatology 38(4 Suppl 1): 618A, 2003

Association of the envelope glycoproteins of influenza virus with liposomes--a model study on viral envelope assembly. Virology 97(1): 212-217, 1979

A novel elisa for the detection of antibodies to hiv 1 envelope glycoproteins based on immobilization of viral glycoproteins in microtiter wells coated with concanavalin a. Journal of Immunological Methods 132(1): 63-72, 1990

A novel enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to HIV-1 envelope glycoproteins based on immobilization of viral glycoproteins in microtiter wells coated with concanavalin A. Journal of Immunological Methods 132(1): 63-71, 1990

Characterization of nucleocapsid-M protein interaction in murine coronavirus. Advances in Experimental Medicine and Biology 494: 577-582, 2001

Specific interaction between coronavirus leader RNA and nucleocapsid protein. Journal of Virology 62(11): 4288-4295, 1988

Viral O-GalNAc peptide epitopes: a novel potential target in viral envelope glycoproteins. Reviews in Medical Virology 26(1): 34-48, 2016