+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation of genomic clones homologous to transcribed sequences from human X chromosome



Isolation of genomic clones homologous to transcribed sequences from human X chromosome



Somatic Cell and Molecular Genetics 10(6): 561-571



Several X chromosome DNA clones homologous to transcribed sequences were isolated from a human X chromosome library. The clones were selected for their ability to hybridize either with 32P-labeled human cDNA in the presence of an excess of unlabeled human repetitive DNA or with mouse fibroblast cDNA. The X chromosome specificity of these sequences was demonstrated by two criteria: A dosage effect was seen when the clones were hybridized to Southern blots of DNA from 1X and 5X cells, and they hybridized to DNA from mouse-human hybrid cells containing only the human X chromosome. The presence of transcribed sequences in these X clones was detected by hybridization with mouse cDNA or with human cDNA in the presence of unlabeled human repetitive sequences, by identifying restriction fragments which hybridize with cDNA but not with human repetitive DNA, and by hybridization with poly A+ RNA on Northern blots. These clones were mapped on the human X chromosomes using a panel of mouse-human somatic cell hybrids carrying various translocated human X chromosomes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 043483243

Download citation: RISBibTeXText

PMID: 6594767

DOI: 10.1007/bf01535221


Related references

Isolation of nonrepetitive human complementary dna clones for identification of transcribed chromosome specific dna sequences. American Journal of Human Genetics 33(6): 47A, 1981

Isolation and characterization of transcribed sequences from a chromosome 16 hn-cDNA library and the physical mapping of genes and transcribed sequences using a high-resolution somatic cell panel of human chromosome 16. Genomics 20(2): 169-175, 1994

Isolation and characterization of genomic moose Dna clones containing sequences homologous to tRnas and 5S rRna. Nucleic Acids Research 11(14): 4809-4821, 1983

Isolation and characterization of genomic mouse DNA clones containing sequences homologous to tRNAs and 5S rRNA. Nucleic Acids Research 11(14): 4809-4821, 1983

The isolation of transcribed sequences from the human x chromosome. Cytogenetics & Cell Genetics 40(1-4): 721, 1985

Identification and isolation of transcribed human X chromosome DNA sequences. Nucleic Acids Research 11(22): 7961-7979, 1983

Isolation and localization of transcribed sequences on human chromosome 22. Cytogenetics and Cell Genetics 71(1): 81-85, 1995

Properties of random human complementary dna clones homologous to dna sequences from chromosome 7. Cytogenetics & Cell Genetics 37(1-4): 407-408, 1984

Isolation and characteristics of an ordered library of transcribed sequences of human chromosome 19 from hybrid human-hamster cells. Bioorganicheskaia Khimiia 20(8-9): 919-931, 1994

Isolation and characterization of human random complementary dna clones homologous to dna from the x chromosome. Somatic Cell & Molecular Genetics 10(4): 385-398, 1984

Isolation and characterization of human random cDNA clones homologous to DNA from the X chromosome. Somatic Cell and Molecular Genetics 10(4): 385-397, 1984

Isolation of new genes mapping to chromosome 12p evaluation of an arrayed hncDNA library with human transcribed 12p sequences. Cytogenetics & Cell Genetics 61(4): 252, 1992

Isolation of human x chromosomal clones homologous to sex related banded krait minor satellite sequences. American Journal of Human Genetics 37(4 Suppl.): A125, 1985

Isolation of a series of HLA class I clones from a human chromosome 6 genomic library. Molecular Biology and Medicine 2(1): 1-14, 1984

An efficient method for isolating putative promoters and 5'-transcribed sequences from large genomic clones. Genome Research 6(4): 327-335, 1996