EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Modification of the bacteriophage vector M13mp2: introduction of new restriction sites for cloning


Modification of the bacteriophage vector M13mp2: introduction of new restriction sites for cloning



Gene 15(2-3): 167-176



ISSN/ISBN: 0378-1119

PMID: 6271640

DOI: 10.1016/0378-1119(81)90126-8

The construction of two new derivatives of the bacteriophage cloning vector M13mp2 is described. One derivative, mWJ22, contains a new HindIII site while the other, mWJ43, contains a new BamHI site. These new sites were both introduced at the EcoRI site at amino acid five of the 145 amino acid-long fragment of Escherichia coli beta-galactosidase within the phage. The new restriction sites do not disrupt the blue color detection system of M13mp2; therefore insertion of cloned fragments results in colorless plaques on indicator plates for the new derivatives.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 043667630

Download citation: RISBibTeXText

Related references

A versatile prokaryotic cloning vector with six dual restriction enzyme sites in the polylinker facilitates efficient subcloning into vectors with unique cloning sites. Plasmid 40(2): 164-168, 1998

Preparation of Bacteriophage λ DNA Cleaved with Two Restriction Enzymes for Use as a Cloning Vector. Csh Protocols 2006(1), 2006

Cosmid vector pCpG and plasmid vector pKNUN1, and their use for cloning DNA sequences adjacent to sites for rare cutting restriction endonucleases. Nucleic Acids Research 17(9): 3603, 1989

Cosmid vector pCpG and plasmid vector pKNUNl, and their use for cloning DNA sequences adjacent to sites for rare cutting restriction endonucleases. Nucleic Acids Research 17(9): 3603-3603, 1989

Preparation of Bacteriophage λ DNA Cleaved with a Single Restriction Enzyme for Use as a Cloning Vector. Csh Protocols 2006(1), 2006

Duplication of a region in the multiple cloning site of a plasmid vector to enhance cloning-mediated addition of restriction sites to a DNA fragment. Biotechniques 23(5): 814-816, 1997

The restriction mapping of c gene deletions in Streptomyces bacteriophage phi C31 and their use in cloning vector development. Gene 22(2-3): 167-174, 1983

A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene 10(3): 249-259, 1980

Introduction of unique restriction sites into m 13 lac vector phage by base alkylation. Hoppe-Seyler's Zeitschrift fuer Physiologische Chemie 358(10): 1208-1209, 1977

Plasmid pKC7: a vector containing ten restriction endonuclease sites suitable for cloning DNA segments. Gene 7(1): 79-82, 1979

Plasmid pkc 7 a vector containing 10 restriction endo nuclease sites suitable for cloning dna segments. Gene (amsterdam): 79-82, 1979

Site-directed modification of adenoviral vector with combined DNA assembly and restriction-ligation cloning. Journal of Biotechnology 307: 193-201, 2020

PSDTV vector: a modification of the pBluescript SK+ plasmid in order to perform PCR-fragments TA-cloning using Eam1105I restriction endonuclease. Molecular Biology Reports 39(5): 6133-6139, 2012

Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control: I. DNA fragment insertion at the lacZ EcoRI restriction site. Molecular and General Genetics: Mgg 170(2): 161-169, 1979

Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites. Gene 51(2-3): 275-280, 1987