+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Monoclonal antibodies against distinct determinants of histone H5 bind to chromatin



Monoclonal antibodies against distinct determinants of histone H5 bind to chromatin



Biochemistry 23(15): 3459-3466



A series of monoclonal antibodies specific for distinguishable epitopes in chromosomal protein histone H5 were obtained from mice immunized with either free H5 or H5 . RNA complexes. The antibodies elicited by H5 could be distinguished from those elicited by H5 . RNA by their binding to native or acid-denatured H5, by their interaction with the globular region of H5, and by their cross-reactivity with H1o. The specificity of the antibodies was assessed by enzyme-linked immunosorbent assay (ELISA) and immunoblotting experiments. The antibodies could distinguish between H5 and the closely related histones H1 and H1o. The binding of some of the antibodies to the antigens was dependent on the type of assay used, suggesting nonrandom binding of the antigen to the solid supports used in ELISA and immunoblotting. Competitive ELISA experiments indicate that 8 of the 11 antibodies characterized bind to distinct epitopes. Three monoclonal antibodies bind to epitopes which are in close spatial proximity, causing mutual steric hindrance. The monoclonal antibodies bind to nuclei of fixed cells and to isolated chromatin, indicating that the epitopes are present both in the purified protein and in chromatin-complexed H5. These monoclonal antibodies can be used to study the organization of distinct regions of histones H5 and H1o in chromatin and chromosomes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 043677022

Download citation: RISBibTeXText

PMID: 6205690

DOI: 10.1021/bi00310a012


Related references

Molecular specificity of l 2 monoclonal antibodies that bind to carbohydrate determinants of neural cell adhesion molecules and their resemblance to other monoclonal antibodies recognizing the myelin associated glycoprotein. Brain Research 385(2): 237-244, 1986

Purification of anti-histone-H1 antibodies and their use in measuring histone determinants in chromatin by radioimmunoadsorbance. Biochemical and Biophysical Research Communications 68(3): 718-723, 1976

Two distinct methods analyzing chromatin structure using centrifugation and antibodies to modified histone H3: both provide similar chromatin states of the Rit1/Bcl11b gene. Biochemical and Biophysical Research Communications 313(3): 489-495, 2003

Idiotypic determinants of monoclonal antibodies that bind to 3-fucosyllactosamine. Journal of Immunology 136(7): 2562-2567, 1986

Nephritogenic anti dna monoclonal antibodies bind to the glomerulus via two distinct mechanisms. Journal of the American Society of Nephrology 2(3): 565, 1991

Blockade of NK cell lysis is a property of monoclonal antibodies that bind to distinct regions of T-200. Journal of Immunology 131(4): 1742-1747, 1983

Monoclonal antibodies that bind to distinct epitopes on Fc gamma RI are able to trigger receptor function. Journal of Immunology 143(5): 1650-1655, 1989

Monoclonal antibodies that bind to distinct epitopes on FccRI are able to trigger receptor function. Journal of Immunology 143: 50-5, 1989

Monoclonal antibodies bind distinct classes of hemocytes in the moth Pseudoplusia includens. Journal of Insect Physiology 45(2): 113-126, 2003

Monoclonal antibodies against histone H5. Epitope mapping and binding to chromatin. Journal of Biological Chemistry 260(26): 14379-14386, 1985

Mono clonal antibodies against distinct determinants of histone h 5. Journal Of Cell Biologypart 2: 385a, 1983

Characterization of monoclonal antibodies to histone 2B. Localization of epitopes and analysis of binding to chromatin. European Journal of Biochemistry 157(3): 513-521, 1986

Histone demethylase UTX and chromatin remodeler BRM bind directly to CBP and modulate acetylation of histone H3 lysine 27. Molecular and Cellular Biology 32(12): 2323-2334, 2013

Antibodies from patients with systemic lupus erythematosus and drug-induced lupus bind determinants on histone 5 (H5). Molecular Immunology 27(8): 701-711, 1990

Monoclonal antibodies against Manduca sexta hemocytes bind Aedes aegypti hemocytes: characterization of six monoclonal antibodies that bind hemocytes from both species. Developmental and Comparative Immunology 19(6): 451-461, 1995