EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Multiple molecular forms of acid-stable plasmin inhibitor derived from human urinary trypsin inhibitor


Multiple molecular forms of acid-stable plasmin inhibitor derived from human urinary trypsin inhibitor



Enzyme 30(4): 252-258



ISSN/ISBN: 0013-9432

PMID: 6653545

It was found that cyanogen bromide (BrCN) treatment of the highly purified human urinary trypsin inhibitors (H-UTI; specific activity 1,897 U/mg protein, and L-UTI; specific activity 1,850 U/mg protein) readily produced new plasmin inhibitors with almost no loss of UTI activity. Five multiple forms of chemically cleaved inhibitors (UTIB-I, UTIB-II, UTIB-III, UTIB-IV and UTIB-V) could be isolated from BrCN-treated L-UTI by isoelectric focusing and gel filtration. These inhibitors were very acid-stable and their isoelectric points (pI) were 4.5, 4.6, 4.9, 5.1 and 6.4, respectively. The molecular weights by SDS-polyacrylamide gel electrophoresis were almost the same at about 23,000 +/- 3,000. Although these inhibitors showed both anti-plasmin and anti-trypsin activities, much higher anti-plasmin/anti-trypsin activities were observed in the cleaved inhibitors than in the parent UTI. They competitively inhibited human plasmin with Ki values of 3.0-4.1 X 10(-8) mol/l (H-D-Val-Leu-Lys-pNA substrate).

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 043697667

Download citation: RISBibTeXText

Related references

Inhibition of plasmin by a small molecular weight inhibitor derived from human inter alpha trypsin inhibitor. Thrombosis Research 13(3): 563-568, 1978

Differential expression of inter alpha trypsin inhibitor and its active fragment acid stable trypsin plasmin inhibitor astpi immunoreactivity in the liver. Fibrinolysis 4(Suppl. 3): 55, 1990

Kunitz-type proteinase inhibitors derived by limited proteolysis of the inter-alpha-trypsin inhibitor, IV. The amino acid sequence of the human urinary trypsin inhibitor isolated by affinity chromatography. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 362(10): 1351-1355, 1981

About degradation products of the inter-alpha-trypsin inhibitor in serum. I. The inter-alpha-trypsin inhibitor as precursor of the acid stable trypsin-plasmin-inhibitor of the serum. Klinische Wochenschrift 55(7): 337-342, 1977

Kinetics of plasmin ec 3.4.21.7 inhibition in the presence of a synthetic tri peptide substrate the reaction with pancreatic trypsin inhibitor and 2 forms of alpha 2 plasmin inhibitor. Biochemical Journal 199(1): 121-128, 1981

Kinetics of plasmin inhibition in the presence of a synthetic tripeptide substrate. The reaction with pancreatic trypsin inhibitor and two forms of alpha 2-plasmin inhibitor. Biochemical Journal 199(1): 121-127, 1981

Low molecular weight trypsin-plasmin inhibitors isolated from papain treated urinary trypsin inhibitor. Thrombosis and Haemostasis 47(1): 14-18, 1982

Production by human lung cells of trypsin and plasmin inhibitors differing from a 1 anti trypsin a 2 macro globulin c 1 esterase inhibitor and inter alpha trypsin inhibitor. Thrombosis and Haemostasis 38(1): 78, 1977

Different molecular forms of acid-stable trypsin inhibitor in normal and uremic plasma. Clinica Chimica Acta; International Journal of Clinical Chemistry 190(3): 277-281, 1990

Quantitative studies on the total plasmin and the trypsin inhibitor of human blood serum; methods for the titration of total plasmin and of trypsin inhibitor. Journal of Experimental Medicine 89(3): 295-308, 1949

Multiple forms of cancer related urinary proteinase inhibitor edc 1 and relationship to normal inter alpha trypsin inhibitor related urinary proteinase inhibitor hi 30. Proceedings of the American Association for Cancer Research Annual Meeting 33: 30, 1992

Kunitz-type proteinase inhibitors derived by limited proteolysis of the inter-alpha-trypsin inhibitor, V. Attachments of carbohydrates in the human urinary trypsin inhibitor isolated by affinity chromatography. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 362(10): 1357-1362, 1981

Quantitative studies on the total plasmin and the trypsin inhibitor of human blood serum; variations in the blood concentration of total plasmin and of trypsin inhibitor in streptococcal diseases with special reference to rheumatic fever. Journal of Experimental Medicine 89(3): 309-324, 1949

Acid-stable inhibitor of trypsin, plasmin and kallikrein from rabbit blood serum. Voprosy Meditsinskoi Khimii 12(3): 325-327, 1966

Kunitz-type proteinase inhibitors derived by limited proteolysis of the inter-alpha-trypsin inhibitor, VII. Determination of the amino-acid sequence of the trypsin-released inhibitor from bovine inter-alpha-trypsin inhibitor. Hoppe-Seyler's Zeitschrift für Physiologische Chemie 364(12): 1679-1687, 1983