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Mycoplasma mastitis of cattle. 12. The suitability of Mycoplasma isolation, indirect hemagglutination and film inhibition for the diagnosis of Mycoplasma bovis infection



Mycoplasma mastitis of cattle. 12. The suitability of Mycoplasma isolation, indirect hemagglutination and film inhibition for the diagnosis of Mycoplasma bovis infection



Archiv für Experimentelle Veterinarmedizin 37(3): 383-400



Recovery of Mycoplasma bovis from milk and detection of antibody in blood serum samples were consistently successful in weekly examination, using indirect haemagglutination and an inhibition test on six cows experimentally infected by intracisternal inoculation of the pathogen. In naturally infected cows, M. bovis was regularly isolated from the milk of mastitic cows, and antibodies were detected in blood serum samples with both tests from one week after the onset of disease. M. bovis was excreted sporadically in the milk of healthy cows, but positive reactions in serum samples to both tests were found only in a few cases. Antibodies were also detected in serum samples of experimental animals before infection and of cows which did not yield M. bovis from the milk. Negative and inconsistent results were often obtained with both tests when applied to whey samples of naturally infected cows.

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Accession: 043706015

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PMID: 6351782


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