+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Nucleoli of diploid cell strains. Thei normal ultrastructure and the effects of toyocamycin and actinomycin D

Nucleoli of diploid cell strains. Thei normal ultrastructure and the effects of toyocamycin and actinomycin D

Journal of Cell Biology 49(3): 785-802

Nucleoli of cultured Chinese hamster or mouse cells in early passages had a loosely reticular substructure. Within the reticulum small, irregularly shaped, light fibrillar zones occurred which were contiguous with denser fibrillar zones. These denser zones appeared to be connected in some places to the particulate material which composed the mass of the nucleolus. Generally, electron-transparent spaces separated the particulate zones from the fibrillar areas. Treatment with toyocamycin, an agent which is reported to cause a blockage in the processing of ribosomal RNA, greatly inhibited the accumulation of newly synthesized RNA in the cytoplasm, as monitored by radioautography. Toyocamycin treatment caused the gradual disappearance of the granules from the particulate region of the nucleoli, and resulted ultimately in the nucleoli appearing homogeneously fibrillar. Actinomycin D treatment, which inhibited virtually all RNA synthesis, caused a segregation, and finally a disaggregation, of nucleolar components.

(PDF emailed within 1 workday: $29.90)

Accession: 043767366

Download citation: RISBibTeXText

PMID: 5104724

Related references

Effect of actinomycin d on the function and ultrastructure of nucleoli of a swine embryo kidney cell culture. Biologicheskie Nauki (Moscow) (5): 55-61, 1985

Difference in effects of proflavine and actinomycin D on mammalian cell nucleoli. Cancer Research 31(12): 1915-1922, 1971

Effects of actinomycin D, toyocamycin and cycloheximide on the synthesis of low-molecular-weight nuclear RNAs in HeLa cells. European Journal of Biochemistry 35(2): 350-356, 1973

The fine structure of the nucleoli of normal and Actinomycin D-treated Amoeba proteus. Journal of Ultrastructure Research, 23: 260-271, 1968

The Effects Of Actinomycin D On The Ultrastructure Of The Nucleus Of The Amphibian Embryonic Cell. Journal of Cell Biology 21: 245-252, 1964

Radiosensitivities of ten apparently normal human diploid fibroblast strains to cell killing, G2-phase chromosomal aberrations, and cell cycle delay. Cancer Research 48(16): 4535-4538, 1988

Fibrillar centers in the nucleoli of a PEK cell culture after administration of actinomycin D. Tsitologiia 24(2): 137-143, 1982

Are all normal diploid human cell strains alike? Relevance to carcinogenic mechanisms in vitro. Experimental Cell Biology 50(5): 266-270, 1982

Influence of toyocamycin on avian leukemia myeloblasts: cell growth, ultrastructure, RNA synthesis, and elaboration of BAI strain A virus. Cancer Research 30(3): 753-762, 1970

Simplified quantitative assay of effects of adriamycin and actinomycin d on tumor nucleoli in vivo. Federation Proceedings 36(3): 303, 1977

Differences in the internal structure of nucleoli of diploid and non-diploid transformed or neoplastic cells in vitro. Experimental Cell Research 40(1): 188-192, 1965

In vitro variability of the number of nucleoli in the clones of human diploid fibroblasts its connection with cell division potential and cell morphology. Tsitologiya i Genetika 18(6): 408-412, 1984

Morphologic and metabolic alterations in hepatic cell nucleoli induced by varying doses of actinomycin D. Cancer Research 29(1): 124-135, 1969