Nucleotide sequence of the promoter and NH2-terminal signal peptide region of Bacillus subtilis alpha-amylase gene cloned in pUB110

Ohmura, K.; Yamazaki, H.; Takeichi, Y.; Nakayama, A.; Otozai, K.; Yamane, K.; Yamasaki, M.; Tamura, G.

Biochemical and Biophysical Research Communications 112(2): 678-683


ISSN/ISBN: 0006-291X
PMID: 6189486
DOI: 10.1016/0006-291x(83)91516-4
Accession: 043768326

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The nucleotide sequence of the promotor and NH2-terminal signal peptide region of the alpha-amylase gene derived from the alpha-amylase hyperproducing strain B. subtilis NA64 was determined. DNA sequences of the NH2-terminal region of the mature alpha-amylase, 41 amino acid residues of the signal peptide, a Shine-Dalgarno sequence (AGGAG), a potential RNA polymerase recognition site (TTGAAA), and a potential Pribnow box (AAGTAA) were identified. The DNA sequence was quite different from that of the alpha-amylase gene of B. amyloliquefaciens.