+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Acid-base fractions separated from Streblus asper leaf ethanolic extract exhibited antibacterial, antioxidant, anti-acetylcholinesterase, and neuroprotective activities



Acid-base fractions separated from Streblus asper leaf ethanolic extract exhibited antibacterial, antioxidant, anti-acetylcholinesterase, and neuroprotective activities



Bmc Complementary and Alternative Medicine 18(1): 223



Streblus asper is a well-known plant native to Southeast Asia. Different parts of the plant have been traditionally used for various medicinal purposes. However, there is very little scientific evidence reporting its therapeutic benefits for potential treatment of Alzheimer's disease (AD). The study aimed to evaluate antibacterial, antioxidant, acetylcholinesterase (AChE) inhibition, and neuroprotective properties of S. asper leaf extracts with the primary objective of enhancing therapeutic applications and facilitating activity-guided isolation of the active chemical constituents. The leaves of S. asper were extracted in ethanol and subsequently fractionated into neutral, acid and base fractions. The phytochemical constituents of each fraction were analyzed using GC-MS. The antibacterial activity was evaluated using a broth microdilution method. The antioxidant activity was determined using DPPH and ABTS radical scavenging assays. The neuroprotective activity against glutamate-induced toxicity was tested on hippocampal neuronal HT22 cell line by evaluating the cell viability using MTT assay. The AChE inhibitory activity was screened by thin-layer chromatography (TLC) bioautographic method. The partition of the S. asper ethanolic leaf extract yielded the highest mass of phytochemical constitutions in the neutral fraction and the lowest in the basic fraction. Amongst the three fractions, the acidic fraction showed the strongest antibacterial activity against gram-positive bacteria. The antioxidant activities of three fractions were found in the order of acidic > basic > neutral, whereas the decreasing order of neuroprotective activity was neutral > basic > acidic. TLC bioautography revealed one component in the neutral fraction exhibited anti-AChE activity. While in the acid fraction, two components showed inhibitory activity against AChE. GC-MS analysis of three fractions showed the presence of major phytochemical constituents including terpenoids, steroids, phenolics, fatty acids, and lipidic plant hormone. Our findings have demonstrated the therapeutic potential of three fractions extracted from S. asper leaves as a promising natural source for neuroprotective agents with additional actions of antibacterials and antioxidants, along with AChE inhibitors that will benefit in the development of new natural compounds in therapies against AD.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 043923370

Download citation: RISBibTeXText

PMID: 30041641

DOI: 10.1186/s12906-018-2288-4


Related references

Evaluation Of The Anti-Mycobacterial, Anti-Oxidative And Anti-Proliferative Activities Of Rubia Cordifolia Ethanolic Leaf Extract Sub-Fractions In Human Lymphocytes And Neutrophils. African Journal of Traditional, Complementary and Alternative medicines 14(5): 181-187, 2017

Anti-inflammatory and antioxidant activities of ethanolic leaf extract of Brownlowia tersa L Kosterm. Oriental Pharmacy and Experimental Medicine 13(3): 181-189, 2013

Anti-inflammatory effect of Streblus asper leaf extract in rats and its modulation on inflammation-associated genes expression in RAW 264.7 macrophage cells. Journal of Ethnopharmacology 124(3): 566-570, 2009

Antioxidant and acetylcholinesterase inhibitory activities of leaf extract and fractions of Albizia adianthifolia (Schumach) W.F. Wright. Journal of Basic and Clinical Physiology and Pharmacology 28(2): 143-148, 2017

Antimicrobial activity of Streblus asper leaf extract. Phytotherapy Research 15(2): 119-121, 2001

Ethanolic extract of Streblus asper leaves protects against glutamate-induced toxicity in HT22 hippocampal neuronal cells and extends lifespan of Caenorhabditis elegans. Bmc Complementary and Alternative Medicine 17(1): 551, 2017

Crude extract and fractions from Eugenia uniflora Linn leaves showed anti-inflammatory, antioxidant, and antibacterial activities. Bmc Complementary and Alternative Medicine 18(1): 84, 2018

Effect of Streblus asper leaf extract on selected anaerobic bacteria. Acta Horticulturae (680): 177-181, 2005

Microstructure and Texture of Milk Coagulum of Streblus asper Leaf Extract. International Journal of Food Properties 15(5): 933-941, 2012

Effects of Streblus asper leaf extract on the biofilm formation of subgingival pathogens. South African Journal of Botany 94: 1-5, 2014

UPLC-Q-Orbitrap-MS 2 analysis of Moringa oleifera leaf extract and its antioxidant, antibacterial and anti-inflammatory activities. Natural Product Research 2019: 1-5, 2019

Evaluation of antibacterial, antioxidant and DNA protective capacity of Chenopodium album's ethanolic leaf extract. Chemosphere 90(2): 374-379, 2013

Antioxidant, Antibacterial, and Cytotoxic Activities of the Ethanolic Origanum vulgare Extract and Its Major Constituents. Oxidative Medicine and Cellular Longevity 2016: 1404505, 2016

Anti-HBV activities of Streblus asper and constituents of its roots. Fitoterapia 83(4): 643-649, 2012

In vitro antibacterial efficacy of crude ethanolic leaf extract and alkaloidal fractions of phyllanthus amarus on URO-pathogens. 2013