+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Precursor ribosomal ribonucleic acid and ribosome accumulation in vivo during the recovery of Salmonella typhimurium from thermal injury



Precursor ribosomal ribonucleic acid and ribosome accumulation in vivo during the recovery of Salmonella typhimurium from thermal injury



Journal of Bacteriology 107(1): 134-142



When cells of S. typhimurium were heated at 48 C for 30 min in phosphate buffer (pH 6.0), they became sensitive to Levine Eosin Methylene Blue Agar containing 2% NaCl (EMB-NaCl). The inoculation of injured cells into fresh growth medium supported the return of their normal tolerance to EMB-NaCl within 6 hr. The fractionation of ribosomal ribonucleic acid (rRNA) from unheated and heat-injured cells by polyacrylamide gel electrophoresis demonstrated that after injury the 16S RNA species was totally degraded and the 23S RNA was partially degraded. Sucrose gradient analysis demonstrated that after injury the 30S ribosomal subunit was totally destroyed and the sedimentation coefficient of the 50S particle was decreased to 47S. During the recovery of cells from thermal injury, four species of rRNA accumulated which were demonstrated to have the following sedimentation coefficients: 16, 17, 23, and 24S. Under identical recovery conditions, 22, 26, and 28S precursors of the 30S ribosomal subunit and 31 and 48S precursors of the 50S ribosomal subunit accumulated along with both the 30 and 50S mature particles. The addition of chloramphenicol to the recovery medium inhibited both the maturation of 17S RNA and the production of mature 30S ribosomal subunits, but permitted the accumulation of a single 22S precursor particle. Chloramphenicol did not affect either the maturation of 24S RNA or the mechanism of formation of 50S ribosomal subunits during recovery. Very little old ribosomal protein was associated with the new rRNA synthesized during recovery. New ribosomal proteins were synthesized during recovery and they were found associated with the new rRNA in ribosomal particles. The rate-limiting step in the recovery of S. typhimurium from thermal injury was in the maturation of the newly synthesized rRNA.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 044001576

Download citation: RISBibTeXText

PMID: 4935315


Related references

Ribosomal rna and ribosome re synthesis in vivo during the recovery of salmonella typhimurium from thermal injury. Bacteriological Proceedings 71: 124, 1971

Thermal injury and recovery in salmonella typhimurium 7136 the in vivo degradation and re synthesis of ribosomes and ribosomal rna. Dissertation Abstracts International B Sciences & Engineering 32(3): 1731-B-1732-B, 1971

Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium. Infection and Immunity 8(3): 395-400, 1973

Induction of immunoenhancing factors for murine splenocyte cultures by Salmonella typhimurium ribosome and ribonucleic acid extracts. Infection and Immunity 32(3): 1123-1127, 1981

Involvement of ribosomal ribonucleic acid operons in Salmonella typhimurium chromosomal rearrangements. Journal of Bacteriology 143(1): 492-498, 1980

Ribosomal ribonucleic acid isolated from Salmonella typhimurium: absence of the intact 23S species. Journal of Bacteriology 139(3): 842-849, 1979

Electron microscopic mapping of secondary structures in bacterial 16S and 23S ribosomal ribonucleic acid and 30S precursor ribosomal ribonucleic acid. Journal of Bacteriology 141(1): 365-373, 1980

Requirements of Salmonella typhimurium for recovery from thermal injury. Journal of Bacteriology 105(2): 512-518, 1971

Lipid biosynthesis during the recovery of Salmonella typhimurium from thermal injury. Canadian Journal of Microbiology 18(7): 1015-1021, 1972

Thermal injury and recovery of Salmonella typhimurium and its effect on enumeration procedures. Applied Microbiology 18(3): 332-336, 1969

Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in Anacystis nidulans. Biochemical Journal 129(1): 135-140, 1972

Regeneration of ribosomes and ribosomal ribonucleic acid during repair of thermal injury to Staphylococcus. Journal of Bacteriology 94(4): 1082-1087, 1967

Ribonucleic acid splicing in Neurospora Mitochondria: secondary structure of the 35S ribosomal precursor ribonucleic acid investigated by digestion with ribonuclease III and by electron microscopy. Biochemistry 20(10): 2836-2842, 1981

Studies on the formation of transfer ribonucleic acid-ribosome complexes. VII. The role of the 3'-hydroxyl-terminal end of transfer ribonucleic acid for interaction with ribosomes and ribosomal subunits. Journal of Biological Chemistry 245(6): 1497-1503, 1970

Studies on the formation of transfer ribonucleic acid-ribosome complexes. I. The effect of streptomycin and ribosomal dissociation on 14-C-aminoacyl transfer ribonucleic acid binding to ribosomes. Journal of Biological Chemistry 241(2): 367-372, 1966