Preparation of 125I-labeled native DNA for use in radioimmunoassays for anti-native-DNA antibodies

Keiser, H.

Arthritis and Rheumatism 16(4): 468-470

1973


ISSN/ISBN: 2326-5191
PMID: 4541814
DOI: 10.1002/art.1780160406
Accession: 044013529

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
A method is described for the in vitro labeling of calf thymus native Dna (n DNA) with 125I. The 125I-calf thymus n Dna produced by this method is suitable for use in the Millipore filter assay for antibodies to n Dna in sera of patients with systemic lupus erythematosus and gives results equivalent to those obtained with 3H-He La cell n Dna labeled in vivo.

Preparation of 125I-labeled native DNA for use in radioimmunoassays for anti-native-DNA antibodies