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Preparation of lyophilized abnormal hemoglobin control for cellulose acetate electrophoresis


, : Preparation of lyophilized abnormal hemoglobin control for cellulose acetate electrophoresis. American Journal of Clinical Pathology 74(1): 64-67

A process for the preparation of stable lyophilized normal and abnormal hemoglobin controls for alkaline cellulose acetate electrophoresis is described. The hemoglobins were converted to cyanmethemoglobin derivatives with K3Fe(CN)6 and KCN. Sucrose, 100 g/l, was added to stabilize the preparation for lyophilization and storage and to increase the rate of reconstitution. Lyophilized preparations were stable with no loss of resolution for nine months of use in the laboratory and for two weeks of accelerated aging at 37 C. The materials appeared to have an extended shelf-life of at least two years at 5 C. The controls reconstituted quickly, usually within a minute, and could be stored at 5 C for three months with only a slight increase in band streaking. The lyophilized products appeared to be suitable to assist in the development of a quality-control system in hemoglobin screening programs employing cellulose acetate electrophoresis.

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Accession: 044014600

PMID: 7395816

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Schneider S.; Zilow E.; Linderkamp O., 1987: Estimation of deformability of neonatal and adult leukocytes via micropipette aspiration. Pediatric Research 22(2): 219

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Popp W., 1977: The problems of toxicity determination of waste waters by a bacterial test. Bayer Landesanst Wasserforsch/ Muenchner Beitraege Zur Abwasser-, Fischerei- Und Flussbiologie, Band 28 Behandlung Von Industrieabwaessern (Munich Reports on Waste Fish And River Biology, Vol 28 Treatments Of Industrial Wastes ) Seminar Munich, W Ger , 1975 (In Ger ) 327p Illus R Oldenbourg Verlag: Munich, W Ger Isbn 3-486-20551-X 239-246