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Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety

Preparation of radioactive Tay-Sachs ganglioside labeled in the sialic acid moiety

Journal of Lipid Research 11(2): 144-149

ISSN/ISBN: 0022-2275

PMID: 5418476

A procedure is described for the preparation of Tay-Sachs ganglioside specifically labeled in the sialic acid portion of the molecule. Rat brain gangliosides were labeled biosynthetically by the intracranial injection of N-acetyl-(3)H-D-mannosamine. Radioactive gangliosides were isolated and selectively degraded with bacterial neuraminidase and rat liver beta-galactosidase to Tay-Sachs ganglioside-(3)H. Radioactivity in the labeled product was confined to the N-acetyl-neuraminic acid portion of the molecule.

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Accession: 044014927

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