Pressure-fixation method of transferring cells from polycarbonate filters to glass slides
Oud, P.S.; Zahniser, D.J.; Garcia, G.L.; van Boekel, M.C.; Haag, D.J.; Hermkens, H.G.; Pahlplatz, M.M.; Vooijs, P.G.; Herman, C.J.
Analytical and Quantitative Cytology 6(2): 131-138
ISSN/ISBN: 0190-0471 PMID: 6380364 Accession: 044022061
A technique for the preparation of cytology slides is presented by which cells collected on a polycarbonate membrane filter are transferred to a slide by means of simultaneous pressure and fixation. Using cervical samples as a model, the influence of the filtration rate, filter pore size and duration of pressure application on cell recovery was analyzed. The present version of the preparation procedure uses manual techniques that define the operating characteristics of a fully automated procedure.