Section 45
Chapter 44,051

Production of group C streptococcus phage-associated lysin and the preparation of Streptococcus pyogenes protoplast membranes

Wheeler, J.; Holland, J.; Terry, J.M.; Blainey, J.D.

Journal of General Microbiology 120(1): 27-33


ISSN/ISBN: 0022-1287
PMID: 7012275
DOI: 10.1099/00221287-120-1-27
Accession: 044050768

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Optimum conditions of pH, temperature and multiplicity of bacteriophage infection for the production of phage-associated lysin from Group C streptococci were defined. Lysin obtained by the optimum method was used to make protoplast membranes of Group A type 12 streptococci. These membranes consistently contained very low concentrations of rhamnose, indicating minimal cell wall contamination. The development of such a reproducible technique for the preparation of protoplast membranes will permit more meaningful studies on the involvement of these membranes in the pathogenesis of acute post-streptococcal glomerulonephritis.

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