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Production, purification and chemical properties of an antistaphylococcal agent produced by Staphylococcus epidermidis



Production, purification and chemical properties of an antistaphylococcal agent produced by Staphylococcus epidermidis



Journal of General Microbiology 127(2): 377-384



An antibacterial agent produced by Staphylococcus epidermidis strain 5 was isolated from culture supernatants; its spectrum of activity is restricted to staphylococci and micrococci. The bacteriocin-like substance was purified to homogeneity by column chromatography on Servachrome XAD-2, CM-Sephadex C-25 and Sephadex G-50. Chemical analysis showed it to be a peptide with a molecular weight of about 6000 and an isoelectric point of about 10.5. Purified material was rapidly inactivated by trypsin, chymotrypsin and pronase and it was relatively heat-stable. After acidic hydrolysis 10 amino acid residues and two unidentified ninhydrin-positive substances were detected. Production of the bacteriocin-like substance was not inducible with mitomycin C.

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Accession: 044051618

Download citation: RISBibTeXText

PMID: 7343644

DOI: 10.1099/00221287-127-2-377



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