Section 45
Chapter 44,105

Purification of DNA-dependent RNA polymerase Ii from Saccharomyces cerevisiae

Bitter, G.A.

Analytical Biochemistry 128(2): 294-301


ISSN/ISBN: 0003-2697
PMID: 6342468
DOI: 10.1016/0003-2697(83)90378-0
Accession: 044104487

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A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, and chromatography on DEAE-cellulose, DEAE-Sephadex, and phosphocellulose. The procedure may be completed in 2.5 days and the resultant enzyme is judged to be greater than 90% pure.

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