Quantitative laser-nephelometric detection of rheumatoid factor, using IgG coated latex particles

Husmann, K.; Lakomek, H.J.; Richter, O.; Jacobi, E.; Krüskemper, H.L.

Journal of Clinical Chemistry and Clinical Biochemistry 19(11): 1125-1130


ISSN/ISBN: 0340-076X
PMID: 7310334
Accession: 044116529

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The laser-nephelometric test (Latex RF test) detects rheumatoid factor activity, and is based on immune complex formation between IgG coated latex particles and rheumatoid factor. This test requires little time and gives qualitative results in good precision. The latex nephelometric test and the frequently used agglutination tests (Waaler-Rose, Latex agglutination test) give the same qualitative results. In the quantitative assay higher nephelometric results correlate on average with higher titers in the Waaler-Rose and Latex agglutination test. The high standard deviation could be explained by the fact that other factors (IgG rheumatoid factor, IgG and IgG-IGM immune complexes), in addition to the IgM rheumatoid factor concentration, influence the immune complex formation in vitro.