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RNA sequences of opposite polarities transcribed from a large part of vaccinia virus genome are accumulated in the cytoplasm



RNA sequences of opposite polarities transcribed from a large part of vaccinia virus genome are accumulated in the cytoplasm



Acta Virologica 24(6): 451-454



Labelled vaccinia virus DNA was used in saturation-hybridization experiments with RNA extracted from virus-infected cells. An excess of "late" cytoplasmic RNA converted 45% of DNA into DNA-RNA hybrids, whereas 17% of DNA could be converted into hybrids by RNA extracted from purified nuclei. RNA-RNA hybrids obtained from cytoplasmic RNA by self-annealing and ribonuclease treatment, were melted and used in hybridization with DNA: 36% of DNA was hybridized at the maximal concentration used.

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Accession: 044120725

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PMID: 6111206


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