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Radioimmunologic identification of prostaglandins produced by serum-stimulated mouse embryo palate mesenchyme cells



Radioimmunologic identification of prostaglandins produced by serum-stimulated mouse embryo palate mesenchyme cells



Biochimica et Biophysica Acta 712(2): 408-411



High-performance liquid chromatography and radioimmunoassay were used to identify the prostaglandins synthesized by mouse embryo palate mesenchyme cells. Serum stimulated the release of several different metabolites of arachidonic acid including 6-ketoprostaglandin F1 alpha (the stable product of prostacyclin, prostaglandin I2), prostaglandin E2 and prostaglandin F2 alpha. Compared to control cells, the serum-stimulated cells produce elevated levels of prostaglandin E2 (36-fold), 6-ketoprostaglandin F1 alpha (15-fold) and prostaglandin F2 alpha (7-fold). The acetylenic analogue of arachidonic acid, 5,8,11,14-eicosatetraynoic acid prevented this accelerated synthesis.

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Accession: 044129048

Download citation: RISBibTeXText

PMID: 6812642

DOI: 10.1016/0005-2760(82)90360-5


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