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Chapter 44,140

Rapid restriction mapping of DNA cloned in lambda phage vectors

Rackwitz, H.R.; Zehetner, G.; Frischauf, A.M.; Lehrach, H.

Gene 30(1-3): 195-200

1984

ISSN/ISBN: 0378-1119
PMID: 6096214
DOI: 10.1016/0378-1119(84)90120-3
Accession: 044139658
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A protocol for the rapid restriction mapping of phage lambda clones has been developed. Partial digestion products are selectively labelled at the right or left cohesive lambda DNA termini by hybridisation with [32P]oligonucleotides complementary to the single-stranded cos ends. After gel electrophoresis and autoradiography, the restriction map can be directly determined from the "ladder" of partial digestion products.

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