+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Rapid, sensitive and fully automated high-performance liquid chromatographic assay with fluorescence detection for sulmazole and metabolites



Rapid, sensitive and fully automated high-performance liquid chromatographic assay with fluorescence detection for sulmazole and metabolites



Journal of Chromatography 278(2): 347-357



Sulmazole (2-[(2-methoxy-4-methylsulfinyl)phenyl]-3H-imidazo [4,5-b] pyridine; AR-L 115 BS) and two metabolites (sulfide, sulfone) were quantified from directly injected body fluids (plasma, urine, bile) after high-performance liquid chromatographic separation. No internal standard is needed, which is particularly advantageous when fluorescence detection is established. After automated pre-column enrichment on Corasil C18 (37-50 microns), the parent compound and biotransformation products could be backflushed and chromatographed on ODS-Hypersil (5 microns) with a mixture of 0.075 mol/l phosphate buffer-acetonitrile (2:1), an elution rate of 2.0 ml/min and fluorimetric detection (lambda ex = 330 nm; lambda em = 370 nm). A hydroxylated metabolite of sulmazole which occurs preferentially in urine (and bile) can be quantified in the above-mentioned solvent system diluted 1:1 with water, but with different fluorescence characteristics (lambda ex = 345 nm; lambda em = 515 nm). The assay was linear in the range 8-1000 ng/ml. The lower limit of detection was about 8 ng/ml or 80 pg with coefficients of variation between 0.4 and 5.8% for sulmazole.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 044140110

Download citation: RISBibTeXText

PMID: 6668315

DOI: 10.1016/s0378-4347(00)84794-5


Related references

A rapid and sensitive high-performance liquid chromatographic assay for 6-mercaptopurine metabolites in red blood cells. Analytical Biochemistry 144(2): 514-521, 1985

Rapid and sensitive on-line precolumn purification and high-performance liquid chromatographic assay for disulfiram and its metabolites. Journal of Chromatography 378(2): 419-429, 1986

Sensitive high-performance liquid chromatographic assay for norfloxacin utilizing fluorescence detection. Journal of Chromatography B Biomedical Applications 663(2): 379-384, 1995

Sensitive and specific high-performance liquid chromatographic assay with ultraviolet detection for the determination of cocaine and its metabolites in rat plasma. Journal of Chromatography B 703(1-2): 129-138, 1997

Sensitive and specific high-performance liquid chromatographic assay with ultraviolet detection for the determination of cocaine and its metabolites in rat plasma. Journal of Chromatography. B Biomedical Sciences and Applications 703(1-2): 129-138, 1997

Rapid and sensitive analysis of parishin and its metabolites in rat plasma using ultra high performance liquid chromatography-fluorescence detection. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 973c: 104-109, 2014

High-performance liquid chromatographic method for rapid and highly sensitive determination of histidine using postcolumn fluorescence detection with o-phthaldialdehyde. Journal of Chromatography B 718(2): 235-241, 1998

High-performance liquid chromatographic assay with fluorescence detection for the simultaneous measurement of carboxylate and lactone forms of irinotecan and three metabolites in human plasma. Journal of Chromatography B 788(1): 65-74, 2003

Fully automated high-performance liquid chromatographic method for the determination of carzelesin (U-80,244) and metabolites (U-76,073 and U-76,074) in human plasma. Journal of Chromatography B Biomedical Applications 652(1): 51-58, 1994

Rapid and sensitive assay of monoamine transmitters and their metabolites in biological samples using high performance liquid chromatography with electrochemical detection. Sheng Li Xue Bao 39(4): 412-419, 1987

Application of a high-performance liquid chromatographic fluorescence method for the rapid determination of alpha-tocopherol in the plasma of cattle and pigs and its comparison with direct fluorescence and high-performance liquid chromatography-ultraviolet detection methods. Journal of Chromatography 178(2): 525-531, 1979

Fully automated high-performance liquid chromatographic assay for the analysis of free catecholamines in urine. Journal of Chromatography 530(1): 11-18, 1990

Simultaneous determination of gemfibrozil and its metabolites in plasma and urine by a fully automated high performance liquid chromatographic system. Biomedical Chromatography 5(2): 68-73, 1991

Efficient high-performance liquid chromatographic assay for the simultaneous determination of metoprolol and two main metabolites in human urine by solid-phase extraction and fluorescence detection. Journal Of Chromatography B. 696(1): 69-74, 1997

Sensitive chiral high-performance liquid chromatographic determination of anthelmintic flubendazole and its phase I metabolites in blood plasma using UV photodiode-array and fluorescence detection Application to pharmacokinetic studies in sheep. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 876(1): 89-96, 2008