EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Regulation of heat-shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells


, : Regulation of heat-shock genes: a DNA sequence upstream of Drosophila hsp70 genes is essential for their induction in monkey cells. Embo Journal 1(10): 1279-1285

Heat-shock genes coding for heat-shock protein 70 (HSP70) in Drosophila melanogaster were subcloned into an SV40/plasmid recombinant capable of replication in permissive monkey COS cells. Following transfection of COS cells, no significant amount of Drosophila hsp70 RNA was detected at 37 degrees C. In contrast, a heat-shock at 43 degrees C or arsenite poisoning at 37 degrees C induced the massive production of Drosophila hsp70 RNA of correct size and faithful 5' ends. After heat-shock, the efficiency of hsp70 transcription in COS cells containing 2-4 X 10(4) gene copies was found to be 15-30% of that measured in Drosophila, on a per gene basis. By testing a series of 5' deletion mutants in this inducible transcription assay it was found that a sequence less than 70 bp long, directly upstream of the hsp70 gene, was essential for the heat or arsenite induction of transcription.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 044174318

PMID: 6821333

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Anon, 1986: Milestone upjohn centennial. American Pharmacy 26(3): 86

Regli, L.; Held, M.C.; Anderson, R.E.; Meyer, F.B., 1996: Nitric oxide synthase inhibition by L-NAME prevents brain acidosis during focal cerebral ischemia in rabbits. This experiment examined the effects of nitric oxide (NO) synthase inhibition on brain intracellular pH, regional cortical blood flow, and NADH fluorescence before and during 3 h of focal cerebral ischemia using in vivo fluorescence imaging. Thirt...

Nesbit, R.M.; Labardini, M.M., 1967: Infantile prostatism. Michigan Medicine 66(3): 172-176

Limberi, S.; Markou, N.; Sakayianni, K.; Vourliotou, A.; Kremastinou, F.; Savari, E.; Gregorakos, L., 2003: Coronary artery disease and upper abdominal surgery: impact of anesthesia on perioperative myocardial ischemia. Background/Aims: Some papers claim that epidural anesthesia and analgesia lowers the incidence of perioperative ischemic events and may have a favorable effect on perioperative cardiac morbidity and mortality. We studied the effect of epidural ane...

Nappi, F.; Schneider, R.; Zolotukhin, A.; Smulevitch, S.; Michalowski, D.; Bear, J.; Felber, B.K.; Pavlakis, G.N., 2001: Identification of a novel posttranscriptional regulatory element by using a rev- and RRE-mutated human immunodeficiency virus type 1 DNA proviral clone as a molecular trap. Human immunodeficiency virus (HIV) and all other lentiviruses utilize the essential viral protein Rev, which binds to RRE RNA, to export their unspliced and partially spliced mRNAs from the nucleus. We used a rev- and RRE-defective HIV type 1 (HIV...

Tang, P. F.; Chu, Y. H.; Chen, S. L.; Chang, Y. L., 2001: Control mechanisms of multidirectional volitional stepping in young adults A preliminary report. This study investigated the relationship between the center of pressure (COP) and muscle activities of the lower extremities of healthy young adults during voluntary forward, sideway, and backward stepping. Three males and three females (age=21.3+...

McFarland, D. ., 1981: The Oxford companion to animal behaviour. This book is in the form of an illustrated mini-encyclopedia, containing over 200 articles of various lengths arranged alphabetically. There are 2 taxonomic indices, one of English names, the other of scientific names, referring to those articles...

Harper K., 2005: Geomorphic evidence of late stage convergence-parallel extension in an arc-continent collision zone, East Timor. Abstracts - Geological Society of Australia 80: 9

Sripraram, D., 2001: Optimal planning method for logging operation operated by mobile tower yarders. The main objective of this thesis was to create the optimal planning method for varying size tower yarders by determining the optimum size of the tower yarder, the optimum length of cable span and the optimum lateral skidding distance which is sui...

Joy, Melanie S.; Hollar, Kimberly D.; Neyhart, Clara D.; Hogan, Susan L.; Dupuis, Robert E.; Finn, William F.; Dooley, Mary Anne, 2002: Assessment of risk factors for bone disease in renal transplant recipients. Background: Renal allograft recipients are at increased risk of osteoporosis and fractures because of their multiple risk factors for reduced bone mineral density (BMD). Objective: To retrospectively assess the risk factors that may predispose ren...