+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Regulation of heme metabolism and cytochrome P-450 levels in primary culture of rat hepatocytes in a defined medium

, : Regulation of heme metabolism and cytochrome P-450 levels in primary culture of rat hepatocytes in a defined medium. Biochemical Pharmacology 33(4): 565-569

Liver cells were prepared from adult Sprague-Dawley rats and used for the determination of delta-aminolevulinic acid synthetase (ALAS) activity and cytochrome P-450 concentrations at different time intervals in tissue culture in a serum-free synthetic medium. During the first 24 hr in culture, the level of cytochrome P-450 decreased to 30-40% of the level in isolated liver cells from untreated animals. The disappearance of cytochrome P-450 was especially fast in hepatocytes obtained from female phenobarbital-treated rats where only 40% of the original cytochrome P-450 was present after 2 hr in culture and 80% had disappeared in 2 days. The activity of ALAS increased 3- to 4-fold when measured 2 hr after plating, and it reached the maximum level in 19-24 hr when its activity was about eight times the original activity. In 2-4 days in culture, the activity of ALAS was four to five times above the original level. When the amount of delta-aminolevulinic acid (ALA) in the medium was increased from 1 to 100 microM, a decrease in ALAS was obtained, but no significant increase in cytochrome P-450 level was observed. Addition of heme to the medium gave a dose-dependent decrease in the activity of ALAS. Our data indicate that during the first 24 hr in culture the increase of ALAS activity was prevented by exogenous heme. This effect may be due to inhibition of the catalytic activity, suppression of the synthesis of the enzyme, or accelerated breakdown of the enzyme by heme.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 044174324

PMID: 6704172

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Gunasekera, Lalith; Bonila, Julio, 2001: Alligator weed: Tasty vegetable in Australian backyards?. The present distribution of alligator weed (Alternanthera philoxeroides (Mart.) Griseb.) in Australia is cause for considerable concern, earning it a place among the top 20 weeds of National Significance. It is considered one of the worst aquatic...

Shelley, W. B.; Shelley, E. D., 1984: Nicotinic-acid-responsive photosensitivity. A white man 59 years old with a poor diet and a history of two-thirds gastrectomy became sensitive to summer sunlight but had no persistent dermatitis or other sign of pellagra. After 10 days on nicotinic acid, 100 mg twice daily, he had no reacti...

Romanov V. U., 1980: The absorption of flushing fluids during the exposure of water-bearing horizons. Pages 106-112 1980

Berlin, Nathaniel I., 1960: Effect of chronic ionizing radiation on the life span of man. B B Watson, Ed The delayed effects of whole-body radiation 11-13

Vikturina, V.P.; Gorelova, L.N.; Polianskaia, Z.M., 1976: Standardizing the work of x-ray room personnnel. Vestnik Rentgenologii i Radiologii (): 70-74

Greenhalgh, M. E., 1965: Shelduck numbers on the Ribble estuary. Bird Study, 12: 255-256

Anonymous, 1994: Rebuilding NH3 plants. Many large ammonia plants in the former Soviet Union and elsewhere in Eastern Europe need to be revamped. Their energy consumption should be reduced and their capacity increased within a reasonably short pay-back time. Some possible solutions are...

Bozzaro, S.; Roseman, S., 1983: Adhesion of dictyostelium discoideum cells to carbohydrates immobilized in poly acrylamide gels 1. evidence for 3 sugar specific cell surface receptors. D. discoideum cells appear to be able to recognize particular carbohydrate prosthetic groups at different stages in their life cycle. A previously developed model system (consisting of polyacrylamide gels containing putative ligands covalently lin...

Diaz Gomez, M. I.; Valles, E.; Fanelli, S. L.; Delgado De Layno, A. M. A.; Castro, G. D.; Castro, J. A., 2001: Alcohol induction of liver nuclear ethanol and N-nitrosodimethylamine metabolism to reactive metabolites. Toxicology Letters (Shannon) 123(Supplement 1): 77-78, September 1st

Yan, Jiangli; Zhu, Chongjie; Pu, Guogong; Wang, Erkang, 1993: Differential pulse stripping voltammetry of cancerostatic 5-fluorouracil and determination in blood serum. Bioelectrochemistry & Bioenergetics 29(3): 347-355