EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Regulation of sugar transport in cultured diploid human skin fibroblasts


Regulation of sugar transport in cultured diploid human skin fibroblasts



Journal of Cellular Physiology 112(3): 367-372



ISSN/ISBN: 0021-9541

PMID: 6182150

DOI: 10.1002/jcp.1041120309

The regulation of hexose transport under glucose-starvation conditions was studied in cultured human skin fibroblasts. Glucose starvation enhanced the transport of 2-DG and 3-0-methyl-D-glucose (3-OMG) but not of L-glucose. Glucose-starvation enhanced transport was inhibited by cytochalasin B (10 microM). The starvation-induced change in 2-DG transport was due to an increase in the Vmax of both the high and low affinity transport sites (2.8- and 2.4-fold, respectively) with no effect on their Kms. The presence of 5.55 mM glucose, fructose, or L-glucose in the medium resulted in transport increases similar to those seen in glucose-starved cells, while the presence of 5.55 mM glucose, mannose, or 3-OMG repressed 2-DG transport. Glucose-starvation enhancement of 2-DG transport was blocked by cycloheximide (20 micrograms/ml) but not by actinomycin D (0.03 microgram/ml) or alpha-amanitin (3.5 microM). Readdition of glucose (5.55 mM) for six hours to glucose-starved cells led to a rapid decrease in hexose transport that could be blocked by cycloheximide but not actinomycin D. Although readdition of 3-OMG to glucose-starved cells had little effect on reversing the transport increases, glucose plus 3-OMG were more effective than glucose alone. Serum containing cultures (10% v/v) of glucose-fed or glucose-starved cells exhibited rapid decreases in 2-DG transport when exposed to glucose-containing serum-free medium. These decreases were prevented by employing glucose-free, serum-free medium. The data indicate that hexose transport regulation in cultured human fibroblasts involves protein synthesis of hexose carriers balanced by interactions of glucose with a regulatory protein(s) and glucose metabolism as they affect the regulation and/or turnover of the carrier molecules.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 044175749

Download citation: RISBibTeXText

Related references

Regulation of insulin binding and stimulation of sugar transport in cultured human fibroblasts by sugar levels in the culture medium. Archives of Biochemistry and Biophysics 234(2): 559-566, 1984

Enhanced insulin stimulation of sugar transport and DNA synthesis by glucocorticoids in cultured human skin fibroblasts. Archives of Biochemistry and Biophysics 226(2): 498-505, 1983

Factors affecting glucose starvation enhancement of sugar transport in cultured human skin fibroblasts. Journal of Cell Biology 83(2 Part 2): 292A, 1979

Reciprocal regulation of glucose and glutamine utilization by cultured human diploid fibroblasts. Journal of Cellular Physiology 95(1): 41-48, 1978

Transport of cystine and cysteine and cell growth in cultured human diploid fibroblasts: effect of glutamate and homocysteate. Journal of Cellular Physiology 112(2): 265-272, 1982

Glucose transport and metabolism in cultured human skin fibroblasts. Biochimica et Biophysica Acta 762(2): 198-204, 1983

Down-regulation of the glucocorticoid receptor by dexamethasone in cultured human skin fibroblasts: implications for the regulation of aromatase activity. Journal of Clinical Endocrinology and Metabolism 66(5): 1029-1036, 1988

The effect of insulin on hexose transport in cultured human skin fibroblasts. Journal of Cell Biology 79(2 Part 2): 203A, 1978

Stimulation of hexose transport in cultured human skin fibroblasts by insulin. Journal of Cellular Physiology 99(3): 313-318, 1979

The effect of glibenclamide on insulin stimulated sugar amino acid transport in cultured human fibroblasts. Journal of Cell Biology 95(2 Part 2): 196A, 1982

Immobilized glycolipids from human diploid fibroblasts inhibit DNA synthesis of cultured human fibroblasts. Experimental Cell Research 169(1): 15-24, 1987

The regulation of 3 o methyl d glucose transport by cultured human fibroblasts. Federation Proceedings 46(6): 1982, 1987

Transport and processing of endocytosed lysosomal α-glucosidase in cultured human skin fibroblasts. Febs Journal 158(2): 339-344, 1986

Studies on the effects of in vitro ageing on saturable and nonsaturable sugar uptake in cultured human skin fibroblasts. Gerontology 26(4): 181-187, 1980

Cultured human skin fibroblasts and keratinocytes regulation of cholesterol synthesis. Journal Of Investigative Dermatology: 331, 1982