+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Replication of coronavirus MHV-A59 in sac- cells: determination of the first site of budding of progeny virions

Replication of coronavirus MHV-A59 in sac- cells: determination of the first site of budding of progeny virions

European Journal of Cell Biology 33(2): 281-293

During infection of sac- cells by murine coronavirus MHV A59 the intracellular sites at which progeny virions bud correlate with the distribution of the viral glycoprotein E1. Budding is first detectable by electron microscopy at 6 to 7 hours post infection in small, smooth, perinuclear vesicles and tubules in a region transitional between the rough endoplasmic reticulum and the Golgi apparatus. At later times the rough endoplasmic reticulum becomes the major site of budding and accumulation of progeny virus particles. Indirect immunofluorescence microscopy shows that E1 is confined at 6 hours post infection to the perinuclear region while at later times it also accumulates in the endoplasmic reticulum. At 6 hours post infection the second viral glycoprotein, E2, is distributed throughout the endoplasmic reticulum and is not restricted to the site at which budding begins. Core protein, the third protein in virions, can be detected 2 hours before E1 is detectable and budding begins, and at 6 hours post infection it is distributed throughout the cytosol. We conclude that the time and the site at which the maturation of progeny virions occurs is determined by the accumulation of glycoprotein E1 in intracellular membranes. Only rarely do progeny virions bud directly into the cisternae of the Golgi apparatus but at least some already budded virions are transported to the Golgi apparatus where they occur in structures some of which also contain TPPase, a trans Golgi marker.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 044203435

Download citation: RISBibTeXText

PMID: 6325194

Related references

MHV-JHM infections of rodent neuronal cells: replication and trafficking of structural proteins and progeny virions. Advances in Experimental Medicine and Biology 342: 319-325, 1993

Structure of the 3 progeny virions produced by a budding enveloped phage. Biophysical Journal 45(2 Part 2): 166A, 1984

The intracellular sites of early replication and budding of SARS-coronavirus. Virology 361(2): 304-315, 2007

Culturing the unculturable: human coronavirus HKU1 infects, replicates, and produces progeny virions in human ciliated airway epithelial cell cultures. Journal of Virology 84(21): 11255-11263, 2010

Evidence in thin sections of human mammary adeno carcinoma for possible b type virions and for budding virions. Journal of Cell Biology 55(2 PT 2): 274A, 1972

Coronavirus M proteins accumulate in the Golgi complex beyond the site of virion budding. Journal of Virology 68(10): 6523-6534, 1994

Pseudotype virions formed between mouse hepatitis virus and lactate dehydrogenase-elevating virus (LDV) mediate LDV replication in cells resistant to infection by LDV virions. Journal of Virology 69(7): 4237-4244, 1995

Characterization of the Lipidomic Profile of Human Coronavirus-Infected Cells: Implications for Lipid Metabolism Remodeling upon Coronavirus Replication. Viruses 11(1):, 2019

Transmissible gastroenteritis coronavirus genome packaging signal is located at the 5' end of the genome and promotes viral RNA incorporation into virions in a replication-independent process. Journal of Virology 87(21): 11579-11590, 2013

Surface structure of virions budding from L1210(V) gln- mouse leukemia cells. Virology 75(2): 484-487, 1976

The 1st site of budding of coronavirus a 59 defines a compartment on the transport pathway between the endoplasmic reticulum and the stacks of golgi cisternae. Journal of Cell Biology 99(4 Part 2): 230A, 1984

The vaccinia virus B5 protein requires A34 for efficient intracellular trafficking from the endoplasmic reticulum to the site of wrapping and incorporation into progeny virions. Journal of Virology 82(5): 2161-2169, 2008

HIV virions produced by cells treated with the HIV-1 protease inhibitor KNI-272 do not acquire infectivity after viral budding. Journal of Investigative Medicine 44(3): 252A, 1996

Identification of the budding virions of in vitro cultured l 1210 leukemia cells by immuno electron microscopy. Proceedings of the American Association for Cancer Research 15: 96, 1974

Electron microscopic study on the surfaces of gross virus induced murine leukemia cells and budding virions. Okayama Igakkai Zasshi 90(9-10): 1073-1092, 1978