Section 45
Chapter 44,288

Selective inhibition of f2 RNA translation by sulfhydryl reagents

Singer, R.E.; Conway, T.W.

Archives of Biochemistry and Biophysics 158(1): 257-265


ISSN/ISBN: 0003-9861
PMID: 4580843
DOI: 10.1016/0003-9861(73)90620-6
Accession: 044287404

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Escherichia coli ribosomes containing bound initiation factors were reacted with N-ethylmaleimide (NEM) under conditions where about 20 Nem molecules bind per ribosome. This treatment results in a large reduction in their ability to translate f2 Rna but not late T4 m Rna or polyuridylate. Iodoacetate produces a similar effect. These results indicate that ribosomal factors responsible for m Rna specificity differ appreciably in their sensitivity to inactivation by alkylation and suggest that translational control, dependent on m Rna selectivity, might operate by modifying the same or similar reactive sites.

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