+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Sequence-specific endonuclease Bam HI. Effect of hydrophobic reagents on sequence recognition and catalysis



Sequence-specific endonuclease Bam HI. Effect of hydrophobic reagents on sequence recognition and catalysis



Journal of Biological Chemistry 255(14): 6521-6524



The specificity of cleavage of Bam HI is altered in the presence of hydrophobic reagents, such as glycerol and M2SO. The enzyme with altered specificity, designated Bam HI.1, generated digestion patterns of various DNAs, which were distinct from those generated by Bam HI. Cleavage sites recognized in phiX174 RF DNA in the presence of these hydrophobic reagents are not related to the Bam HI palindrome. Bam HI.1 appears to be an endogenous form of Bam HI that can be expressed by altering the hydrophobicity of the reaction.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 044298925

Download citation: RISBibTeXText

PMID: 6248525


Related references

Effect of hydrophobic reagents on sequence recognition and catalysis of dna by sequence specific endo nuclease bam h i. Federation Proceedings 39(6): ABSTRACT 1414, 1980

DNA sequence recognition by a eukaryotic sequence-specific endonuclease, Endo.SceI, from Saccharomyces cerevisiae. Journal of Biological Chemistry 266(8): 5342-5347, 1991

Enzymatic sequence-specific spin labeling of a DNA fragment containing the recognition sequence of EcoRI endonuclease. Febs Letters 228(1): 33-36, 1988

Sequence-specific endonuclease BamHI: relaxation of sequence recognition. Proceedings of the National Academy of Sciences of the United States of America 79(8): 2432-2436, 1982

Mutational analysis of the function of Gln115 in the EcoRI restriction endonuclease, a critical amino acid for recognition of the inner thymidine residue in the sequence -GAATTC- and for coupling specific DNA binding to catalysis. Journal of Molecular Biology 229(1): 221-234, 1993

Amino acid residues in the GIY-YIG endonuclease II of phage T4 affecting sequence recognition and binding as well as catalysis. Journal of Bacteriology 190(16): 5533-5544, 2008

Dna determinants in sequence-specific recognition by Xm al endonuclease. Nucleic Acids Research 23(17): 3571-3577, 1995

Sequence-specific DNA recognition by the SmaI endonuclease. Journal of Biological Chemistry 270(12): 6496-6504, 1995

DNA determinants in sequence-specific recognition by Xmal endonuclease. Nucleic Acids Research 23(17): 3571-3577, 1995

Relaxation of recognition sequence of specific endonuclease HlndIii. Nucleic Acids Research 14(2): 811-821, 1986

Relaxation of recognition sequence of specific endonuclease HindIII. Nucleic Acids Research 14(2): 811-821, 1986

Restriction endonuclease in acetic acid bacteria. Part II. Purification, properties and recognition sequence of site-specific restriction endonuclease from "Acetobacter xylinus". Agricultural and Biological Chemistry 47(12): 2871-2879, 1983

Restriction endonuclease in acetic acid bacteria. Part III. Purification, properties and recognition sequence of site-specific restriction endonuclease from Acetobacter liquefaciens AJ 2881. Agricultural and Biological Chemistry 48(12): 3027-3034, 1984

Recognition sequence of specific endonuclease BamH.I from Bacillus amyloliquefaciens H. Nature 265(5589): 82-84, 1977

The recognition sequence of site specific endonuclease bbv ii from bacillus brevis 80. Febs Letters 177(1): 23-26, 1984