Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes

Rappold, G.A.; Cremer, T.; Hager, H.D.; Davies, K.E.; Müller, C.R.; Yang, T.

Human Genetics 67(3): 317-325

1984


ISSN/ISBN: 0340-6717
PMID: 6336321
DOI: 10.1007/bf00291361
Accession: 044313713

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Abstract
Two cloned repetitive DNA probes, pXBR and CY1, which bind preferentially to specific regions of the human X and Y chromosome, respectively, were used to study the distribution of the sex chromosomes in human lymphocyte nuclei by in situ hybridization experiments. Our data indicate a large variability of the distances between the sex chromosomes in male and female interphase nuclei. However, the mean distance observed between the X and Y chromosome was significantly smaller than the mean distance observed between the two X-chromosomes. The distribution of distances determined experimentally is compared with three model distributions of distances, and the question of a non-random distribution of sex chromosomes is discussed. Mathematical details of these model distributions are provided in an Appendix to this paper. In the case of a human translocation chromosome (Xqter----Xp22.2::Yq11----Yqter) contained in the Chinese hamster X human hybrid cell line 445 X 393, the binding sites of pXBR and CY1 were found close to each other in most interphase nuclei. These data demonstrate the potential use of chromosome-specific repetitive DNA probes to study the problem of interphase chromosome topography.

Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes