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Specific enzyme immunoassay for human chorionic gonadotropin using antibody to the synthetic peptide corresponding to the carboxyl-terminal region of the beta-subunit of hCG

Furuhashi, Y.; Sekiya, T.; Goto, S.; Kaseki, S.; Tomoda, Y.

Endocrinologia Japonica 29(5): 517-521

1982

ISSN/ISBN: 0013-7219
PMID: 6189707
DOI: 10.1507/endocrj1954.29.517
Accession: 044372576
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A sandwich-type enzyme immunoassay for human chorionic gonadotropin (hCG) was developed with anti hCG-beta antibody-enzyme complex and the immobilized solid-phase antibody which was prepared from the antiserum to the synthetic peptide corresponding to the carboxyl-terminal region of the beta-subunit of hCG (hCG-beta). This assay system showed a high sensitivity equal to the radioimmunoassay and did not cross-react with human luteinizing hormone (hLH), human follicle stimulating hormone (hFSH) or alpha-subunit of hCG (hCG-alpha). The coefficients of variation in within-run and between-run were less than 10% and there was a good correlation between the enzyme immunoassay and the radioimmunoassay of serum sample with a regression equation of y = 1.038 x -0.647 and a correlation coefficient of r = 0.953.

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Related References

Furuhashi, Y.; Sekiya, T.; Goto, S.; Kaseki, S.; Tomoda, Y. 1982: Specific enzyme immunoassay for human chorionic gonadotropin using antibody to the synthetic peptide corresponding to the carboxyl terminal region of the beta subunit of human chorionic gonadotropin Endocrinologia Japonica 29(5): 517-522
Furuhashi, Y.; Sekiya, T.; Goto, S.; Kaseki, S.; Tomoda, Y. 1981: Specific enzyme immunoassay for human chorionic gonadotropin using antibody against the synthetic peptide corresponding to the carboxyl terminal region of human chorionic gonadotropin beta Acta Obstetrica et Gynaecologica Japonica (Japanese Edition) 33(12): 2336
Matsuura, S.; Ohashi, M.; Chen, H.C.; Hodgen, G.D. 1979: A human chorionic gonadotropin-specific antiserum against synthetic peptide analogs to the carboxyl-terminal peptide of its beta-subunit Endocrinology 104(2): 396-401
Kondo, K.; Imagawa, M.; Iwasa, S.; Kitada, C.; Konishi, E.; Suzuki, N.; Yamoto, M.; Nakano, R.; Yoshitake, S.; Ishikawa, E. 1984: A specific and sensitive sandwich enzyme immunoassay for human chorionic gonadotropin using antibodies against the carboxyl-terminal portion of the beta-subunit Clinica Chimica Acta; International Journal of Clinical Chemistry 138(2): 229-235
Matsuura, S.; Chen, H.C.; Hodgen, G.D. 1978: Antibodies to the carboxyl-terminal fragment of human chorionic gonadotropin beta-subunit: characterization of antibody recognition sites using synthetic peptide analogues Biochemistry 17(4): 575-580
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Kan, S.; Iriyama, H.; Yoshio, T.; Ninagawa, T.; Tomoda, Y. 1981: Specific determination of human chorionic gonadotropin using antibodies to the carboxyl terminal nonadeca peptide of human chorionic gonadotropin beta subunit Acta Obstetrica et Gynaecologica Japonica (Japanese Edition) 33(12): 2281
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Suzuki, N.; Konishi, E.; Kondo, K.; Fujita, I.; Ishikawa, E. 1985: A highly sensitive colorimetric sandwich-enzyme immunoassay for human chorionic gonadotropin using specific antibodies against the carboxy-terminal portion of the beta-subunit Clinica Chimica Acta; International Journal of Clinical Chemistry 150(3): 247-253
Furuhashi, M.; Suganuma, N. 2004: Processing of O-linked glycosylation in the chimera consisting of alpha-subunit and carboxyl-terminal peptide of the human chorionic gonadotropin beta-subunit is affected by dimer formation with follicle-stimulating hormone beta-subunit Endocrine Journal 51(1): 53-59
Lentz, S.; Birken, S.; Boime, I. 1983: Post translational modification of the carboxyl terminal region of the beta subunit of human chorionic gonadotropin Federation Proceedings 42(7): Abstract 906
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Okumura, H.; Sudo, T.; Sawai, M. 1979: A specific radio immunoassay of a human chorionic gonadotropin beta subunit in the presence of native human chorionic gonadotropin and immunological biological and chemical characterization of human chorionic gonadotropin and its chemically modified subunits Folia Endocrinologica Japonica 55(1): 38-53