+ Translate

Specificity of the Westerfeld adaptation of the Voges-Proskauer test

, : Specificity of the Westerfeld adaptation of the Voges-Proskauer test. Applied and Environmental Microbiology 44(1): 40-43

Aliphatic chain compounds at least four carbons long with vicinal carbonyl groups in the 2,3 positions were detected by the Westerfeld test. Acetoin, which has one carbonyl group and an adjacent hydroxyl group, gave positive results, but methyl action (3-hydroxy-3-methyl-2-butanone) was negative, and subsequent tests supported the conclusion that acetoin is oxidized to diacetyl by alpha-naphthol during the Westerfeld test in the absence or presence of air. 2,3-Pentanedione and 2,3-heptanedione gave positive results, but equimolar concentrations of these compounds gave maximal absorbancy readings that were only 35% (2,3-pentanedione) and 31% (2,3-heptanedione) of those obtained with diacetyl or acetoin. Negative results were obtained with pyruvic acid, 2,3-butylene glycol, and carbon ring compounds (1,2-cyclohexanedione, alloxan, and 3,4-dihydroxy-3-cyclobutene-1,2-dione). alpha-Naphtho could not be replaced in the test by beta-naphthol, 1,2,3,4,-tetrahydroxy-1-naphthol, or 5,6,7,8-tetrahydroxy-1-naphthol. Creatine could not be replaced by arginine, guanidine . HCl, or guanidinoacetic acid.

(PDF 0-2 workdays service)

Accession: 044374715

PMID: 6751225

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Kijima, K.; Koyahara, I.; Tanabe, S.; Sakaguchi, T., 1971: Studies on the mechanism of the Voges-Proskauer reaction. V. On the structure of the orange color substance produced in the Voges-Proskauer reaction with morpholinoamidine and 1,1-pentamethyleneguanidine. Yakugaku Zasshi 91(11): 1150-1153

Tanabe S.; Sakaguchi T., 1979: Studies on the voges proskauer reaction part 9 a new spectrophotometric determination method for moroxydine by voges proskauer reaction. A simple and specific method for the determination of moroxydine in serum was established by the use of ion-exchange chromatography and a modified Voges-Proskauer colorimetric reaction. Moroxydine over the range of 5-70 .mu.g was determined by the...

Tanabe, S.; Sakaguchi, T., 1979: Studies on the Voges-Proskauer reaction. IX. A new spectrophotometric determination method for moroxydine by Voges-Proskauer reaction. Chemical & Pharmaceutical Bulletin 27(3): 813-815

Szafir, J.J.; Bennett, E.O., 1953: The adaptation of the Voges-Proskauer reaction for the quantitative assay of streptomycin. Science 117(3052): 717-718

Ljutov, V., 1963: Technique Of Voges-Proskauer Test. Acta Pathologica et Microbiologica Scandinavica 58: 325-335

Banerjea, R., 1944: Some observations on Voges-Proskauer test. The Barritt test and the original V.P. test give better results in media containing no phosphates. In a medium containing phosphates, positives tended to become negative after > 2 days' incubation. Even under opt. conditions the original V...

Ljutov, V., 1963: Technic of Voges-Proskauer test. Ljutov's methyl-red test and the Voges-Proskauer test are compared. The type of peptone used in the dextrose-phosphate medium is more important in the VP than in the MR test. Barritt's method for the titration of dextrose-phosphate cultu...

Werkman, C.H., 1930: An Improved Technic For The Voges-Proskauer Test. Journal of Bacteriology 20(2): 121-125

Barry, A.L.; Feeney, K.L., 1967: Two quick methods for Voges-Proskauer test. Two previously described methods for rapidly performing a Voges-Proskauer (VP) test were modified so that results could be obtained even more quickly. On MacConkey agar, colonies of lactose-fermenting, VP-positive organisms usually contain enough...

D.Graaf, H., 1941: A revision of the procedure for the Voges Proskauer test. The recommended medium consists of 2% glucose, 0.1% K2HPO4, 0.05% MgSO4, 0.1% NH4C1 (or (NH4)2SO4) and 1% CaCO3. Incubate for 24 hrs. (or if necessary for 48 hrs.) at 37[degree]C. To 3 drops of the culture soln. in a test tube, add 3 drops of a 4...