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Specificity of the bacteriophage PBS2 induced inhibitor of uracil-DNA glycosylase


, : Specificity of the bacteriophage PBS2 induced inhibitor of uracil-DNA glycosylase. Biochemistry 20(21): 6092-6096

The purified PBS2 phage-coded inhibitor of uracil-DNA glycosylase (Ura-DNA glycosylase) from Bacillus subtilis has been tested for its ability to inhibit this enzyme isolated from other prokaryotic and from eukaryotic sources. In addition, the inhibitor has been assayed for its effect on DNA glycosylases specific for other base residues in DNA. The data indicate that Ura-DNA glycosylases from a variety of sources are equally sensitive to inhibition by the inhibitor. DNA glycosylases specific for base residues in DNA other than uracil are not inhibited by the PBS2-coded inhibitor.

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Accession: 044374736

PMID: 6796110

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