EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Specificity of the transport system for tricarboxylic acid cycle intermediates in renal brush borders


, : Specificity of the transport system for tricarboxylic acid cycle intermediates in renal brush borders. Journal of Membrane Biology 57(1): 73-82

Uptake studies employing renal brush border membranes were used to examine the structural specificity of the TCA cycle intermediate transport system. The kinetics of reciprocal inhibition between succinate and citrate revealed these compounds to be transported by a common mechanism. The Michaelis constant for succinate (0.11 mM) was significantly lower than that of citrate (0.28 mM), indicating that the system has a higher affinity for succinate than for citrate. The specificity of the transport system was determined from the relative inhibitory constants of 40 organic acids on the transport of succinate. The results established that the system is highly specific for 4-carbon, terminal dicarboxylic acids in the trans-configuration, including the major intermediates of the TCA cycle. The system is comparatively insensitive to monocarboxylates. Substitution of one of several polar, non-charged residues on the alpha-carbon of succinate permitted interaction of the substrate with the transport system, but substitutions on both the alpha and beta-carbons did not. The structural specificity of the system is fundamentally different from that of the dicarboxylate and tricarboxylate exchange systems of mitochondria. The role of this transport system in the reabsorption of TCA cycle intermediates from the proximal tubule is discussed.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 044374859

PMID: 7452725

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Pedersen, J.; Schurizek, B.A.; Melsen, N.C.; Juhl, B., 1988: Mini-Trach II and acute tracheostomy. Ugeskrift for Laeger 150(17): 1061-1061

Leighfield K. G., 1999: Coal as an energy resource. Coal has been extracted in Britain since Roman times and output reached its peak in 1913. Extraction under private ownership reached the point at which interaction between adjacent collieries created commercial and safety problems, and ownership w...

Shetty, S.; Cohen, A.L.; Edmond, K.; Ojo, L.; Loo, J.; O'Loughlin, R.; Hajjeh, R., 2010: A systematic review and critical evaluation of invasive Haemophilus influenzae type B disease burden studies in Asia from the last decade: lessons learned for invasive bacterial disease surveillance. In Asia, questions regarding the burden of Haemophilus influenzae type b (Hib) disease have delayed decision-making on introduction of Hib vaccine. However, over the past decade many studies have been published regarding Hib disease burden in Asia...

Kinoshita, H.; Ameno, K.; Sumi, Y.; Kumihashi, M.; Ijiri, I.; Ameno, S.; Kubota, A.; Hishida, S., 2003: Evidence of hexavalent chromium ingestion. We describe the application of histochemical demonstration of chromium in a case of fatal ingestion of potassium dichromate in a suicide attempt. Using 2-(8-quinolylazo)-4,5-di-p-tolylimidazole (QTI), we could demonstrate chromium in the erythrocy...

Levasseur, A.; Saloheimo, M.; Navarro, D.; Andberg, M.; Pontarotti, P.; Kruus, K.; Record, E., 2011: Exploring laccase-like multicopper oxidase genes from the ascomycete Trichoderma reesei: a functional, phylogenetic and evolutionary study. Bmc Biochemistry 11(): 32-32

Pearson, A., 2015: Never say never about our child. Bmj 350(): H1246-H1246

Villafuerte-Gálvez, J.; Curioso, W.H.; Gayoso, O., 2007: Biomedical journals and global poverty: is HINARI a step backwards?. Plos Medicine 4(6): E220-E220

Feldmann, H.J.; Molls, M.; Baumhoer, W.; Müller, R.D.; Sack, H., 1988: Radiation and hyperthermia in the treatment of superficial and semi-deep tumors: heat profile, heat doses, tumor remission. Between August, 1986 and October, 1987, 364 hyperthermia treatments combined with radiotherapy were performed in mostly locally advanced and recurrent tumors. Half of the patients could only be exposed to relatively small radiation doses of 20 to...

Matsushita, M.; Kilpatrick, D.; Shiraki, H.; Liu, Y.; Tateishi, K.; Tsujimura, M.; Endo, Y.; Fujita, T., 2014: Purification, measurement of concentration, and functional complement assay of human ficolins. Ficolins constitute a group of lectins involved in innate immunity. L-Ficolin, H-ficolin, and M-ficolin are present in human serum. The human ficolins differ in carbohydrate-binding specificity, but they have in common the ability to recognize the...

Dallinga, H., 1986: Process and equipment for gentle heating of, in particular, liquid foods. Gentle heat treatment of liquid foods utilizes a medium which is subjected to a defined pressure, which is at least as great as the steam pressure which corresponds to the max. temp. of the heating medium. Therefore, if the heating medium is hot w...