+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
EurekaMag PDF Full Text Supply ServicePDF Full Text Supply Service
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn
Follow on LinkedInFollow on Tumblr

+ Translate

Spectral and electron transfer properties of Sepharose 6MB-immobilized cytochrome c

, : Spectral and electron transfer properties of Sepharose 6MB-immobilized cytochrome c. Journal of Biological Chemistry 258(21): 12835-12841

Cytochrome c has been covalently attached to Sepharose 6MB to study the effects of immobilization on the molecule. A detailed study of the resulting product was conducted based on its three characteristic properties: spectra, oxidation-reduction potential, and biological activity. The spectral properties demonstrated that cytochrome c was essentially the same after attachment. No major conformational changes were indicated. The redox potentials for most samples of immobilized cytochrome c loaded with different amounts of protein were generally 20-25 mV lower than native cytochrome c (270 mV). Heavily loaded samples, however, showed no difference in potential. The Km values for immobilized cytochrome c with cytochrome oxidase and reductase from submitochondrial particles were comparable to the soluble protein. Vmax values are more strongly affected by immobilization, especially for the reductase. It has been demonstrated that the submitochondrial particles cannot penetrate the pores of the support material and therefore only the cytochrome c molecules on the surface are available for reaction. As a support material, Sepharose 6MB, which is CNBr activated, hydrolyzes at a significant rate at the pH of the coupling reaction, and this must be considered in establishing coupling conditions for protein immobilization.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 044375173

PMID: 6313678

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Chander, P.; Singh, Y.; Garg, D. N.; Kapoor, P. K., 2001: Occurrence of Mollicutes in cows with various reproductive disorders. The mycoplasmological examination of cervico-vaginal swabs/vaginal discharges from 136 cows in Haryana, India including 86 with various reproductive disorders (22 anoestrus, 25 repeat breeder, 6 cervicitis, 17 mastitis, 16 abortion/stillbirth) and...

Kocnar, K., 1930: Contribution to the technique of selection after hybridization. Points out that the number of sterile spikelets at the base of the ear varies with the distance apart at which the plants are spaced.

Van Gossum, A.; Portal, B.; Le Moine, O.; Van Gossum, M.; Vanderpas, J. B.; Carpentier, Y.; Neve, J., 1996: Effect of oral selenium supplementation on peroxidative and immunological parameters in patients with alcohol-related cirrhosis. Gastroenterology 110(4 SUPPL ): A1351

D'yakonova I.N.; Tikhomirov A.M., 1990: Effect of monopolar and bipolar stimulation of the acoustic nerve by electrical impulses. The amplitudes of the EPs during monopolar and bipolar stimulation of the acoustic nerve were analysed in the cat auditory cortex. Increasing number of trains of 0.2 and 0.5 msec duration of short (5 .mu.sec) electric pulses were delivered to the...

Sherer R.; Brelje T.; Lackman C.; Louis T.A.; Luskin Hawk R., 1992: Predictive value of hiv related non aids clinical conditions based on cpcra observational data. Viii International Conference on Aids And The Iii Std World Congress Published Abstracts Submitted to The Viii International Conference on Aids And The Iii Std World Congress; Harvard-Amsterdam Conference, Amsterdam, Netherlands, July 19-24, 220p Viii International Congress And The Iii Std World Congress: Amsterdam, Netherlands Paper : 103

Fetter, K.; Van Wilder, Vérie.; Moshelion, M.; Chaumont, Fçois., 2003: Interactions between plasma membrane aquaporins modulate their water channel activity. Plant plasma membrane intrinsic proteins (PIPs) cluster in two evolutionary subgroups, PIP1 and PIP2, with different aquaporin activities when expressed in Xenopus oocytes. Maize ZmPIP1;1 and ZmPIP1;2 do not increase the osmotic water permeability...

Ragan, D.P., 1989: Radiotherapy departmental automation. Cost-effective radiotherapy departmental information systems have been developed to answer a growing need for facility management and clinical research. These systems provide scheduling and management support to improve patient flow, facility util...

Sanphoti, N.; Towprayoon, S.; Chaiprasert, P.; Nopharatana, A., 2006: The effects of leachate recirculation with supplemental water addition on methane production and waste decomposition in a simulated tropical landfill. In order to increase methane production efficiency, leachate recirculation is applied in landfills to increase moisture content and circulate organic matter back into the landfill cell. In the case of tropical landfills, where high temperature and...

Anderson, E. G., 1954: The economic value of herbicides to Canadian agriculture. Estimates of the annual loss due to weeds in U.S.A. vary between $1.8 and 36 thousand mn. Estimated % yield losses are: India 27.8, England 7-20, U.S.A. 10 and Canada 10-20. In Canada in 1952 over $6 mn. was spent on herbicides i.e. more than on f...

Vaughan, T. Wayland; Cole, W. Storrs, 1932: Cretaceous orbitoidal Foraminifera from the Gulf States and Central America. [long dash]ASTERORBIS (p. 611), near Orbitocyclina, type A. rooki* (p. 611), Up. Cretaceous, Mississippi; and Pseudorbitoides israel-skii* (p. 614), Up. Cretaceous, Louisiana (type), Texas, and Guatemala. P. israelskii is considered an index fossi...