+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Stabilization and the cytoplasmic ground substance in detergent-opened cells and a structural and biochemical analysis of its composition

Stabilization and the cytoplasmic ground substance in detergent-opened cells and a structural and biochemical analysis of its composition

Proceedings of the National Academy of Sciences of the United States of America 78(7): 4329-4333

ISSN/ISBN: 0027-8424

PMID: 6945586

DOI: 10.1073/pnas.78.7.4329

Treatment of epithelial BSC-1 cells with low concentrations of the detergent Brij 58 results in partial or complete removal of the plasmalemma and partial extraction of internal membrane-bound organelles without causing massive release of "cytosolic" proteins from the cytoplasmic ground substance. Stereoscopic high-voltage electron microscopy of such extracted and fixed cells demonstrates a system of slender (4-20 nm) strands in a three-dimensional "microtrabecular" arrangement similar to that observed in unextracted whole-mount preparations. Extraction of Brij-extracted cells with Triton X-100 dissolves many of the microtrabecular strands, leaving, as a more stable structure, a characteristic cytoskeletal network composed of various filaments and microtubules. Two-dimensional polyacrylamide gel electrophoresis of 35S-labeled polypeptides performed concurrently with the morphological studies demonstrates that Triton extraction of Brij-extracted cells releases a large number of polypeptides. This release parallels the loss of structural components observed by electron microscopy. Labeling of Brij-extracted cells with heavy meromyosin subfragment 1 decorates actin filaments with characteristic arrowhead complexes which are readily visualized only after subsequent Triton extraction. These observations support the concept that many cytoplasmic proteins are structure-bound and, in addition to the components comprising the cytoskeleton, are structure-forming. We conclude that a metastable association of various proteins of the cytoplasmic ground substance exists whose morphological integrity is maintained, at lest temporarily, after removal of the plasmalemma in solutions containing Brij 58.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 044387857

Download citation: RISBibTeXText

Related references

Further evidence for the existence of a structural network in the cytoplasmic ground substance of living cells. Journal of Cell Biology 103(5 Part 2): 286A, 1986

The composition and the behavior of the ground substance of the pulp in relation to the physicochemical properties of colloidal systems. I. Composition of the ground substance. Hellenika Stomatologika Chronika. Hellenic Stomatological Annals 27(3): 103-106, 1983

Biochemical Composition of Dry Substance of Above-Ground Biomass and RAPE SEEDS. Vestnik of the Kazan State Agrarian University 15(2): 29-34, 2020

Organization of the cytoplasmic ground substance of cultured ovarian granulosa cells. Anatomical Record 196(3): 14A, 1980

The Structure of Cytoplasm in Directly Frozen Cultured Cells. I. Filamentous Meshworks and the Cytoplasmic Ground Substance. The Journal of Cell Biology 99(5): 1655-1668, 1984

The structure of cytoplasm in directly frozen cultured cells. I. Filamentous meshworks and the cytoplasmic ground substance. Journal of Cell Biology 99(5): 1655-1668, 1984

Co-determination of ATP and proteins in Triton X 100 non-ionic detergent-opened monolayer cultured cells. Luminescence: the Journal of Biological and Chemical Luminescence 22(5): 415-419, 2007

Enzymatic detergent composition and method for enzyme stabilization. Official Gazette of the United States Patent and Trademark Office Patents 1187(3): 1942, 1996

Lactobionamide-based fluorinated detergent for functional and structural stabilization of membrane proteins. Methods 180: 19-26, 2020

Organization in the structure of the cytoplasmic ground substance. Sturgess, J M (Ed ) Electron Microscopy , Vol Iii State Of The Art Symposia Ninth International Congress Toronto, Ont , Canada, Aug 1-9, Xxix+699p Illus Microscopical Society Of Canada: Toronto, Ont , Canada ISBN 0-920622-08-9: 627-639, 1978

Structured components of the cytoplasmic ground substance. Proceedings Annual meeting: 7th) 2-5, 1979

Fine structure of cytoplasmic ground substance. Nordisk Medicin 60(37): 1326-1327, 1958

Microtrabecular lattice of the cytoplasmic ground substance. Artifact or reality. Journal of Cell Biology 82(1): 114-139, 1979

Epithelial cell motility: the effect of 2-deoxyglucose on cell migration, ATP production, and the structure of the cytoplasmic ground substance in lamellipodia of epithelial cells in culture. Cell Motility 2(1): 25-46, 1982

Comparison of vibrational spectroscopy to biochemical and flow cytometry methods for analysis of the basic biochemical composition of mammalian cells. Journal of Biomedical Optics 11(6): 064024, 2006