+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Studies on the mechanism of RNA synthesis of a murine coronavirus

Studies on the mechanism of RNA synthesis of a murine coronavirus

Advances in Experimental Medicine and Biology 173: 187-200

The mechanism of viral RNA replication in mouse hepatitis virus (MHV)-infected cells was studied by oligonucleotide mapping of every mRNA. We discovered that an oligonucleotide, No. 10, was localized at the 5'-end of every mRNA, and was not colinear with the sequences of the virion genomic RNA. This result indicates that all of the mRNAs contain a leader sequence which is joined to the body sequences of the mRNAs. We have also studied the structure of the replicative intermediate (RI) RNA in the MHV-infected cells. This RI RNA consists of a single species corresponding to the MHV genomic RNA. No subgenomic RI structures were detected. Furthermore, the nascent RNA chains in the RI structure contained the leader sequences, suggesting that the leader RNA was not added to the mRNA post- transcriptionally , but rather, it was probably synthesized independently and then used as a primer for the synthesis of mRNAs. We have also shown that the poly (A) sequences in the MHV genome were transcribed from the poly (U) sequences present in the negative-strand template. The RNA polymerases involved in the MHV RNA synthesis were also characterized. The early polymerase synthesizes a single negative-stranded, full-length RNA. The late polymerases could be separated into two activities, one synthesizing positive-stranded genomic RNA, and the other synthesizing genomic as well as subgenomic RNAs. Thus, the replication and transcription functions of MHV could probably be separated. A plausible model of MHV replication is presented.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 044439981

Download citation: RISBibTeXText

PMID: 6331110

Related references

Defective-interfering particles of murine coronavirus: mechanism of synthesis of defective viral RNAs. Virology 163(1): 104-111, 1988

Murine coronavirus rna synthesis. Brinton, M A And F X Heinz (Ed ) New Aspects Of Positive-Strand Rna Viruses; Second International Symposium, Vienna, Austria, June 1989 Xxi+383p American Society For Microbiology: Washington, D C , Usa Illus Maps 67-74, 1990

Coronavirus isolates SK and SD from multiple sclerosis patients are serologically related to murine coronaviruses A59 and JHM and human coronavirus OC43, but not to human coronavirus 229E. Journal of Virology 38(1): 231-238, 1981

Apoptosis as a mechanism for tissue damage in murine coronavirus induced retinopathy. Investigative Ophthalmology & Visual Science 37(3): S39, 1996

Hygromycin B inhibits synthesis of murine coronavirus RNA. Antimicrobial Agents and ChemoTherapy 35(12): 2630-2633, 1991

Murine coronavirus 5'-end genomic RNA sequence reveals mechanism of leader-primed transcription. Advances in Experimental Medicine and Biology 218: 73-81, 1987

Inhibition of murine coronavirus RNA synthesis by hydroxyguanidine derivatives. Virus Research 14(1): 57-63, 1989

Two murine coronavirus genes suffice for viral RNA synthesis. Journal of Virology 69(4): 2313-2321, 1995

Expression of murine coronavirus genes 1 and 7 is sufficient for viral RNA synthesis. Advances in Experimental Medicine and Biology 380: 479-484, 1995

Synthesis and subcellular localization of the murine coronavirus nucleocapsid protein. Virology 130(2): 527-532, 1983

Unusual heterogeneity of leader-mRNA fusion in a murine coronavirus: implications for the mechanism of RNA transcription and recombination. Journal of Virology 68(10): 6626-6633, 1994

Synthesis of virus-specific RNA in permeabilized murine coronavirus-infected cells. Virology 166(1): 66-75, 1988

Severe acute respiratory syndrome coronavirus protein 6 accelerates murine hepatitis virus infections by more than one mechanism. Journal of Virology 82(14): 7212-7222, 2008

Severe acute respiratory syndrome coronavirus protein 6 accelerates murine coronavirus infections. Journal of Virology 81(3): 1220-1229, 2007