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Studies on transmissible gastroenteritis of swine. 3. The effect of selective inhibitors of viral replication on a cytopathogenic virus from transmissible gastroenteritis



Studies on transmissible gastroenteritis of swine. 3. The effect of selective inhibitors of viral replication on a cytopathogenic virus from transmissible gastroenteritis



Canadian Journal of Comparative Medicine and Veterinary Science 31(11): 299-302



The effect on the plaque production of the Purdue strain of cytopathogenic virus from transmissible gastroenteritis of swine by 5-bromo-2'-deoxyuridine (BUDR), 5-iodo-2'-deoxyuridine (IUDR), actinomycin-D, puromycin, and amantadine-HCI (Symmetral) has been studied.Amantadine-HCI reduced the plaque-forming units of virus per ml by approximately 98%. Puromycin prevented almost all virus reproduction while actinomycin-D caused approximately a 22% reduction. Both IUDR and BUDR produced approximately a 20% increase in plaque-forming units of virus per ml. Swine testis cells stained with acridine orange early in the course of infection contained brick-red particles in the cytoplasm, indicative of a ribonucleic acid (RNA) type virus.

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Accession: 044445538

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PMID: 4169239


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