+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Suppression of murine retrovirus polypeptide termination: effect of amber suppressor tRNA on the cell-free translation of Rauscher murine leukemia virus, Moloney murine leukemia virus, and Moloney murine sarcoma virus 124 RNA

Suppression of murine retrovirus polypeptide termination: effect of amber suppressor tRNA on the cell-free translation of Rauscher murine leukemia virus, Moloney murine leukemia virus, and Moloney murine sarcoma virus 124 RNA

Journal of Virology 34(2): 464-473

ISSN/ISBN: 0022-538X

PMID: 7373716

The effect of suppressor tRNA's on the cell-free translation of several leukemia and sarcoma virus RNAs was examined. Yeast amber suppressor tRNA (amber tRNA) enhanced the synthesis of the Rauscher murine leukemia virus and clone 1 Moloney murine leukemia virus Pr200(gag-pol) polypeptides by 10- to 45-fold, but at the same time depressed the synthesis of Rauscher murine leukemia virus Pr65(gag) and Moloney murine leukemia virus Pr63(gag). Under suppressor-minus conditions, Moloney murine leukemia virus Pr70(gag) was present as a closely spaced doublet. Amber tRNA stimulated the synthesis of the "upper" Moloney murine leukemia virus Pr70(gag) polypeptide. Yeast ochre suppressor tRNA appeared to be ineffective. Quantitative analyses of the kinetics of viral precursor polypeptide accumulation in the presence of amber tRNA showed that during linear protein synthesis, the increase in accumulated Moloney murine leukemia virus Pr200(gag-pol) coincided closely with the molar loss of Pr63(gag). Enhancement of Pr200(gag-pol) and Pr70(gag) by amber tRNA persisted in the presence of pactamycin, a drug which blocks the initiation of protein synthesis, thus arguing for the addition of amino acids to the C terminus of Pr63(gag) as the mechanism behind the amber tRNA effect. Moloney murine sarcoma virus 124 30S RNA was translated into four major polypeptides, Pr63(gag), P42, P38, and P23. In the presence of amber tRNA, a new polypeptide, Pr67(gag), appeared, whereas Pr63(gag) synthesis was decreased. Quantitative estimates indicated that for every 1 mol of Pr67(gag) which appeared, 1 mol of Pr63(gag) was lost.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 044475035

Download citation: RISBibTeXText

Related references

Cleavage of rauscher murine leukemia virus pr 65g a g by the moloney murine leukemia virus proteolytic activity produces rauscher murine leukemia virus specific but not moloney murine leukemia virus specific 40000 dalton intermediate poly peptide. Journal of General Virology 54(1): 33-38, 1981

Translation of moloney murine leukemia virus and moloney murine sarcoma virus rna in nuclease treated reticulocyte extracts enhancement of the gag pol poly peptide with yeast suppressor transfer rna. Cell 13(1): 189-200, 1978

Restriction of Moloney murine leukemia virus replication in Moloney murine sarcoma virus-infected cells - Restriction de la réplication du virus de la leucémie de Moloney dans les cellules infectées par le virus du sarcome murin de Moloney. Virology (New York) 132(2): 377-389, 1984

Significant differences in integration sites of Moloney murine leukemia virus/Moloney murine sarcoma virus retroviral vector carrying recombinant coagulation factor IX in two human cell lines. Biotechnology Letters 37(5): 991-1001, 2015

Relationship between Moloney murine leukemia and sarcoma virus RNAs: purification and hybridization map of complementary DNAs from defined regions of Moloney murine sarcoma virus 124. Journal of Virology 23(3): 524-532, 1977

Restriction of Moloney murine leukemia virus replication in Moloney murine sarcoma virus-infected cells. Virology 132(2): 377-389, 1984

Vertical transmission of murine leukemia virus moloney and murine sarcoma virus moloney and related problems. Yamamoto, Tadashi And Haruo Sugano (Ed ) Gann Monograph on Cancer Research, No 12 Experimental Leukemogenesis Symposium Tokyo, Japan Nov 13-14, 1970 Viii+321p Illus University Park Press: Baltimore, Md , U S A ; Tokyo, Japan 11-31, 1972

Different regions of the mus dunni ecotropic receptor facilitate entry of moloney murine leukemia virus and rauscher murine leukemia virus. Journal of Cellular Biochemistry Suppl (16 Part C): 126, 1992

T cell tolerance in moloney-murine leukemia virus (M-MuLV) carrier mice: low cytotoxic T lymphocyte precursor frequency and absence of suppressor T cells in carrier mice with Moloney-murine sarcoma (M-MSV)-induced tumors. Journal of Immunology 128(2): 774-779, 1982

Identification of directly infected cells in the bone marrow of neonatal moloney murine leukemia virus-infected mice by use of a moloney murine leukemia virus-based vector. Journal of Virology 73(2): 1617-1623, 1999

A p60 polypeptide in the feline leukemia virus pseudotype of Moloney sarcoma virus with murine leukemia virus p30 antigenic determinants. Proceedings of the National Academy of Sciences of the United States of America 72(6): 2380-2384, 1975

Electrophoretic comparisons of the poly peptide composition of the murine and feline leukemia virus pseudotypes of moloney murine sarcoma virus. Abstracts of the Annual Meeting of the American Society for Microbiology 74: 238, 1974

Enhancement of murine sarcoma virus (Moloney) infection and tumorigenesis in vivo by coinfection with Rauscher leukemia virus. Cancer Research 29(11): 1956-1960, 1969

Moloney murine leukemia virus glyco-gag facilitates xenotropic murine leukemia virus-related virus replication through human APOBEC3-independent mechanisms. Retrovirology 9: 58, 2012

Increased oncogenicity of the murine sarcoma virus (Moloney) by co-infection with murine leukemia viruses. Cancer Research 28(6): 1055-1063, 1968