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Synthesis of the disaccharide 6-O-beta-D-galactopyranosyl-2-acetamido-2-deoxy-D-galactose using immobilized beta-galactosidase



Synthesis of the disaccharide 6-O-beta-D-galactopyranosyl-2-acetamido-2-deoxy-D-galactose using immobilized beta-galactosidase



Biochemical and Biophysical Research Communications 123(1): 8-15



The disaccharide 6-O-beta-D-galactopyranosyl-2-acetamido-2-deoxy-D-galactose has been synthesized by transfer of the beta-D-galactopyranosyl residue from lactose to 2-acetamido-2-deoxy-D-galactose utilizing the transferase activity of beta-galactosidase from E. coli. To make the enzyme reusable, it was applied in an immobilized form covalently bound to Sepharose CL-4B. The yield of the disaccharide was about 20%, calculated on the amount of acetamido-deoxy-D-galactose added. The disaccharide could also be obtained by reversal of the hydrolytic activity of the enzyme, using D-galactose and 2-acetamido-2-deoxy-D-galactose as substrate. The yield in this reaction, however, was only 2-3% under the conditions applied.

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Accession: 044508851

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PMID: 6433921


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